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• W2000264645 abstract "In mutagenicity studies a rapid detection of chromosomal damage in mammalian germ cells would be very valuable. Encouraged by the usefulness of the bone-marrow micronucleus test, we applied an analogous method to the assay of micronuclei induced during meiotic reduction divisions in the adult male rat by X-irradiation. The micronuclei were observed in early post-meiotic cells which were enriched using a transillumination phase-contrast microscopic method. The frequency of micronuclei was scored at various dose levels and at various time intervals. The results indicate a linear increase in frequency of micronuclei 24 h after X-irradiation with doses of 0, 10, 50, 150, 300 and 600 rad. The highest frequency of micronuclei was observed after 900 rad whereas lower frequencies were found after 1200 rad. The lowest dose giving a statistically significant increase above the control level was 50 rad. The stages of meiosis showed different sensitivities to the chromosome-breaking action of X-rays. The maximal incidence of micronuclei was found 18 h after irradiation which was considered to reflect the great radiosensitivity of diakinesis-metaphase I. The anesthetized group of control animals showed a slightly higher frequency of micronuclei than the non-anesthetized control. Potentials of the new method for mutagen testing are discussed." @default.
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• W2000264645 date "1981-03-01" @default.
• W2000264645 modified "2023-10-05" @default.
• W2000264645 title "Mieotic micronuclei induced by X-rays in early spermatids of the rat" @default.
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• W2000264645 doi "https://doi.org/10.1016/0027-5107(81)90091-9" @default.
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