FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000265567> ?p ?o ?g. }

• W2000265567 endingPage "343" @default.
• W2000265567 startingPage "330" @default.
• W2000265567 abstract "A simple, rapid, and reproducible method of fetal hepatoblast purification was established to investigate mechanisms controlling interactions between hepatoblasts and nonparenchymal cells during liver development. Because E-cadherin is exclusively expressed on the cell membrane of hepatoblasts, magnetic beads coated with monoclonal antibodies to an extracellular epitope of its molecule were used to purify hepatoblasts from a cell suspension prepared from 12.5-day fetal mouse livers. The purity and yield in the hepatoblast fraction prepared in our protocol were more than 90% and approximately 30%, respectively. The nonparenchymal fraction rarely contained hepatoblasts; the rate of hepatoblast contamination in this fraction was less than 1%. Separate cultures of these two fractions were compared with cocultures of both fractions. In culture of the hepatoblast fraction, hepatoblasts formed aggregates similar to a bunch of grapes via their loose adhesion, floating in the medium after 24 h, and dissociated into single cells from the aggregates after 120 h of culture. By contrast, in the mixed culture, the majority of hepatoblasts formed multicellular spheroids after 24 h, and these spheroids changed into monolayer cell sheets after 120 h of culture. The cells comprising these monolayer sheets abundantly expressed albumin and carbamoylphosphate synthase I. In the mixed culture, fibroblastic cells also proliferated extensively with spreading on glass slides and surrounded the hepatoblast or hepatocyte colonies. On the other hand, fibroblastic cells spreading on glass slides decreased gradually in cultures of the nonparenchymal cell fraction alone. These findings indicated that the coexistence of hepatoblasts and nonparenchymal cells may be essential for their mutual survival, proliferation, differentiation, and morphogenesis. The conditioned medium of fetal liver cell cultures could partially replace the effects of the nonparenchymal cells on hepatoblasts in vitro. Our isolation protocol for fetal mouse hepatoblasts using immunobeads can greatly facilitate studies on mechanisms of cell-cell interactions during liver development." @default.
• W2000265567 created "2016-06-24" @default.
• W2000265567 creator A5024433221 @default.
• W2000265567 creator A5038201008 @default.
• W2000265567 creator A5050629953 @default.
• W2000265567 creator A5078012581 @default.
• W2000265567 date "2002-10-01" @default.
• W2000265567 modified "2023-10-07" @default.
• W2000265567 title "Purification of Fetal Mouse Hepatoblasts by Magnetic Beads Coated with Monoclonal Anti-E-Cadherin Antibodies and Their In Vitro Culture" @default.
• W2000265567 cites W130358101 @default.
• W2000265567 cites W1677076286 @default.
• W2000265567 cites W1839696315 @default.
• W2000265567 cites W1966571134 @default.
• W2000265567 cites W1972305087 @default.
• W2000265567 cites W1975655671 @default.
• W2000265567 cites W1977928014 @default.
• W2000265567 cites W1982132304 @default.
• W2000265567 cites W1987126158 @default.
• W2000265567 cites W1996134446 @default.
• W2000265567 cites W2001557241 @default.
• W2000265567 cites W2005738900 @default.
• W2000265567 cites W2009303198 @default.
• W2000265567 cites W2015709210 @default.
• W2000265567 cites W2017842650 @default.
• W2000265567 cites W2019477145 @default.
• W2000265567 cites W2023469977 @default.
• W2000265567 cites W2026939185 @default.
• W2000265567 cites W2031896298 @default.
• W2000265567 cites W2033791639 @default.
• W2000265567 cites W2036025816 @default.
• W2000265567 cites W2042428579 @default.
• W2000265567 cites W2045598566 @default.
• W2000265567 cites W2048422129 @default.
• W2000265567 cites W2052497886 @default.
• W2000265567 cites W2060183661 @default.
• W2000265567 cites W2060727698 @default.
• W2000265567 cites W2063529172 @default.
• W2000265567 cites W2067440177 @default.
• W2000265567 cites W2082539950 @default.
• W2000265567 cites W2090288308 @default.
• W2000265567 cites W2091986760 @default.
• W2000265567 cites W2100837269 @default.
• W2000265567 cites W2103815895 @default.
• W2000265567 cites W2106125634 @default.
• W2000265567 cites W2111072594 @default.
• W2000265567 cites W2127828801 @default.
• W2000265567 cites W2157582025 @default.
• W2000265567 cites W2162179363 @default.
• W2000265567 cites W2204831117 @default.
• W2000265567 cites W77039285 @default.
• W2000265567 doi "https://doi.org/10.1006/excr.2002.5615" @default.
• W2000265567 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12243758" @default.
• W2000265567 hasPublicationYear "2002" @default.
• W2000265567 type Work @default.
• W2000265567 sameAs 2000265567 @default.
• W2000265567 citedByCount "94" @default.
• W2000265567 countsByYear W20002655672012 @default.
• W2000265567 countsByYear W20002655672013 @default.
• W2000265567 countsByYear W20002655672014 @default.
• W2000265567 countsByYear W20002655672015 @default.
• W2000265567 countsByYear W20002655672016 @default.
• W2000265567 countsByYear W20002655672017 @default.
• W2000265567 countsByYear W20002655672018 @default.
• W2000265567 countsByYear W20002655672019 @default.
• W2000265567 countsByYear W20002655672020 @default.
• W2000265567 countsByYear W20002655672022 @default.
• W2000265567 crossrefType "journal-article" @default.
• W2000265567 hasAuthorship W2000265567A5024433221 @default.
• W2000265567 hasAuthorship W2000265567A5038201008 @default.
• W2000265567 hasAuthorship W2000265567A5050629953 @default.
• W2000265567 hasAuthorship W2000265567A5078012581 @default.
• W2000265567 hasConcept C159654299 @default.
• W2000265567 hasConcept C203014093 @default.
• W2000265567 hasConcept C28406088 @default.
• W2000265567 hasConcept C542903549 @default.
• W2000265567 hasConcept C54355233 @default.
• W2000265567 hasConcept C81885089 @default.
• W2000265567 hasConcept C86803240 @default.
• W2000265567 hasConcept C95444343 @default.
• W2000265567 hasConceptScore W2000265567C159654299 @default.
• W2000265567 hasConceptScore W2000265567C203014093 @default.
• W2000265567 hasConceptScore W2000265567C28406088 @default.
• W2000265567 hasConceptScore W2000265567C542903549 @default.
• W2000265567 hasConceptScore W2000265567C54355233 @default.
• W2000265567 hasConceptScore W2000265567C81885089 @default.
• W2000265567 hasConceptScore W2000265567C86803240 @default.
• W2000265567 hasConceptScore W2000265567C95444343 @default.
• W2000265567 hasFunder F4320320912 @default.
• W2000265567 hasFunder F4320324704 @default.
• W2000265567 hasIssue "2" @default.
• W2000265567 hasLocation W20002655671 @default.
• W2000265567 hasLocation W20002655672 @default.
• W2000265567 hasOpenAccess W2000265567 @default.
• W2000265567 hasPrimaryLocation W20002655671 @default.
• W2000265567 hasRelatedWork W1986936937 @default.
• W2000265567 hasRelatedWork W1999686187 @default.
• W2000265567 hasRelatedWork W2024392898 @default.
• W2000265567 hasRelatedWork W2028679378 @default.

• next