FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000268323> ?p ?o ?g. }

• W2000268323 endingPage "149" @default.
• W2000268323 startingPage "141" @default.
• W2000268323 abstract "The reproducible photolabeling of the androgen receptor from human skin fibroblasts, using [3H]methyltrienolone (R-1881) as ligand is described. Crude nuclei were irradiated for 2 min using a UV lamp with an emission line at 352 nm and a CuSO4 filter. After KCl extraction, proteins were precipitated with trichloroacetic acid, washed with ether and assayed for radioactivity. Specific binding was determined as the difference in bound radioactivity between cells incubated with [3H]R-1881 +/- a 200-fold excess of unlabeled dihydrotestosterone (DHT). The photolabeled proteins were analyzed on SDS-polyacrylamide gel electrophoresis yielding one peak of 90 kDa and in several cases, one of 43 kDa. These peaks comprised 60 +/- 20% of the saturable binding recovered on the gels. The overall efficiency of photolabeling was between 1 and 5%. The amount of covalently bound radioactivity was proportional to the number of cells used. The labeling was inhibited by R-1881, DHT, the anti-androgens hydroxyflutamide and cyproterone acetate and to a lesser extent by estradiol and progesterone. No covalent attachment of R-1881 to any protein was observed when nuclei from patients with androgen insensitivity were irradiated, whether or not the cells were receptor positive or negative. In conclusion the androgen receptor from human skin fibroblast can be efficiently photolabeled and could be used as a marker to follow receptor purification. The absence of photolabeling of nuclear extracts from receptor-positive androgen-insensitive patients may reflect some abnormality of the receptor." @default.
• W2000268323 created "2016-06-24" @default.
• W2000268323 creator A5000953551 @default.
• W2000268323 creator A5041410079 @default.
• W2000268323 creator A5047783815 @default.
• W2000268323 creator A5063112864 @default.
• W2000268323 creator A5087638882 @default.
• W2000268323 date "1987-12-01" @default.
• W2000268323 modified "2023-10-05" @default.
• W2000268323 title "Photoaffinity labeling of the androgen receptor from human skin fibroblasts" @default.
• W2000268323 cites W1277257610 @default.
• W2000268323 cites W1509804955 @default.
• W2000268323 cites W1525712799 @default.
• W2000268323 cites W1639145419 @default.
• W2000268323 cites W1906197854 @default.
• W2000268323 cites W1966700716 @default.
• W2000268323 cites W1981209718 @default.
• W2000268323 cites W1987290219 @default.
• W2000268323 cites W1997556810 @default.
• W2000268323 cites W2000671476 @default.
• W2000268323 cites W2013098053 @default.
• W2000268323 cites W2030209074 @default.
• W2000268323 cites W2030875360 @default.
• W2000268323 cites W2033962951 @default.
• W2000268323 cites W2036277757 @default.
• W2000268323 cites W2041320907 @default.
• W2000268323 cites W2046171537 @default.
• W2000268323 cites W2061530358 @default.
• W2000268323 cites W2064656340 @default.
• W2000268323 cites W2072221541 @default.
• W2000268323 cites W2074013684 @default.
• W2000268323 cites W2093954013 @default.
• W2000268323 cites W2100837269 @default.
• W2000268323 cites W2100936588 @default.
• W2000268323 cites W2113813883 @default.
• W2000268323 cites W4293247451 @default.
• W2000268323 doi "https://doi.org/10.1016/0303-7207(87)90151-1" @default.
• W2000268323 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3500883" @default.
• W2000268323 hasPublicationYear "1987" @default.
• W2000268323 type Work @default.
• W2000268323 sameAs 2000268323 @default.
• W2000268323 citedByCount "5" @default.
• W2000268323 crossrefType "journal-article" @default.
• W2000268323 hasAuthorship W2000268323A5000953551 @default.
• W2000268323 hasAuthorship W2000268323A5041410079 @default.
• W2000268323 hasAuthorship W2000268323A5047783815 @default.
• W2000268323 hasAuthorship W2000268323A5063112864 @default.
• W2000268323 hasAuthorship W2000268323A5087638882 @default.
• W2000268323 hasConcept C121608353 @default.
• W2000268323 hasConcept C12554922 @default.
• W2000268323 hasConcept C126322002 @default.
• W2000268323 hasConcept C153911025 @default.
• W2000268323 hasConcept C170493617 @default.
• W2000268323 hasConcept C185592680 @default.
• W2000268323 hasConcept C2777155358 @default.
• W2000268323 hasConcept C2777332483 @default.
• W2000268323 hasConcept C2777911890 @default.
• W2000268323 hasConcept C2778581200 @default.
• W2000268323 hasConcept C2780192828 @default.
• W2000268323 hasConcept C2780679169 @default.
• W2000268323 hasConcept C55493867 @default.
• W2000268323 hasConcept C61367390 @default.
• W2000268323 hasConcept C71315377 @default.
• W2000268323 hasConcept C71924100 @default.
• W2000268323 hasConcept C86803240 @default.
• W2000268323 hasConceptScore W2000268323C121608353 @default.
• W2000268323 hasConceptScore W2000268323C12554922 @default.
• W2000268323 hasConceptScore W2000268323C126322002 @default.
• W2000268323 hasConceptScore W2000268323C153911025 @default.
• W2000268323 hasConceptScore W2000268323C170493617 @default.
• W2000268323 hasConceptScore W2000268323C185592680 @default.
• W2000268323 hasConceptScore W2000268323C2777155358 @default.
• W2000268323 hasConceptScore W2000268323C2777332483 @default.
• W2000268323 hasConceptScore W2000268323C2777911890 @default.
• W2000268323 hasConceptScore W2000268323C2778581200 @default.
• W2000268323 hasConceptScore W2000268323C2780192828 @default.
• W2000268323 hasConceptScore W2000268323C2780679169 @default.
• W2000268323 hasConceptScore W2000268323C55493867 @default.
• W2000268323 hasConceptScore W2000268323C61367390 @default.
• W2000268323 hasConceptScore W2000268323C71315377 @default.
• W2000268323 hasConceptScore W2000268323C71924100 @default.
• W2000268323 hasConceptScore W2000268323C86803240 @default.
• W2000268323 hasIssue "2-3" @default.
• W2000268323 hasLocation W20002683231 @default.
• W2000268323 hasLocation W20002683232 @default.
• W2000268323 hasOpenAccess W2000268323 @default.
• W2000268323 hasPrimaryLocation W20002683231 @default.
• W2000268323 hasRelatedWork W1509804955 @default.
• W2000268323 hasRelatedWork W1673311932 @default.
• W2000268323 hasRelatedWork W1982420904 @default.
• W2000268323 hasRelatedWork W2000268323 @default.
• W2000268323 hasRelatedWork W2000671476 @default.
• W2000268323 hasRelatedWork W2018443368 @default.
• W2000268323 hasRelatedWork W2031738663 @default.
• W2000268323 hasRelatedWork W2154662370 @default.
• W2000268323 hasRelatedWork W2312631373 @default.
• W2000268323 hasRelatedWork W2418943170 @default.
• W2000268323 hasVolume "54" @default.

• next