Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000268530> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2000268530 endingPage "143" @default.
- W2000268530 startingPage "141" @default.
- W2000268530 abstract "Interleukin-6 (IL-6) exerts its effects by binding to specific receptors on the cell surface. The IL-6 receptor consists of at least two components, a ligand binding 80 kDa low-affinity component (IL-6R) and a signal-transducing non-ligand binding 130 kDa component (gp130). The presence of soluble forms of these components has been described in both conditioned media and biological fluids. The soluble (s) IL-6R has been shown to enhance the IL-6 sensitivity of several both murine and human IL-6 sensitive cell types. A sensitive and commonly used method for measuring biological IL-6 activity is based on the IL-6 dependent proliferation of the murine hybridoma cell line B9. In this paper, we demonstrate that recombinant (r) human (h) sIL-6R enhances the sensitivity of B9 cells in a dose-dependent manner. The rhsIL-6R enhanced the binding of 125I-rhIL-6 to B9 cells. The rhsIL-6R induced stimulation of B9 proliferation was maximal at 100 ng/ml, even without addition of rhIL-6 and in the presence of anti-hIL-6 antibodies. This may be due to endogenous IL-6 production by the B9 cells, low levels of IL-6 in the fetal calf serum used, or perhaps an IL-6 independent effect by the rhsIL-6R. In conclusion, this and other reports point to the necessity of confirming measured biological activities through the use of neutralizing specific antibodies or parallel measurements in immunochemical assays." @default.
- W2000268530 created "2016-06-24" @default.
- W2000268530 creator A5044709706 @default.
- W2000268530 creator A5045129874 @default.
- W2000268530 creator A5057254606 @default.
- W2000268530 date "1992-02-01" @default.
- W2000268530 modified "2023-09-27" @default.
- W2000268530 title "The response of the IL-6-dependent cell line, B9, to IL-6 is not augmented by recombinant derived purified IL-2" @default.
- W2000268530 cites W1501216109 @default.
- W2000268530 cites W2023746846 @default.
- W2000268530 cites W2040020188 @default.
- W2000268530 cites W2063068982 @default.
- W2000268530 cites W2118024162 @default.
- W2000268530 cites W2122248986 @default.
- W2000268530 cites W2138709393 @default.
- W2000268530 cites W2158991537 @default.
- W2000268530 doi "https://doi.org/10.1016/s0022-1759(12)80039-1" @default.
- W2000268530 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1541837" @default.
- W2000268530 hasPublicationYear "1992" @default.
- W2000268530 type Work @default.
- W2000268530 sameAs 2000268530 @default.
- W2000268530 citedByCount "4" @default.
- W2000268530 crossrefType "journal-article" @default.
- W2000268530 hasAuthorship W2000268530A5044709706 @default.
- W2000268530 hasAuthorship W2000268530A5045129874 @default.
- W2000268530 hasAuthorship W2000268530A5057254606 @default.
- W2000268530 hasConcept C104317684 @default.
- W2000268530 hasConcept C116073593 @default.
- W2000268530 hasConcept C116569031 @default.
- W2000268530 hasConcept C11988809 @default.
- W2000268530 hasConcept C126037660 @default.
- W2000268530 hasConcept C134018914 @default.
- W2000268530 hasConcept C153911025 @default.
- W2000268530 hasConcept C159654299 @default.
- W2000268530 hasConcept C16613235 @default.
- W2000268530 hasConcept C170493617 @default.
- W2000268530 hasConcept C185592680 @default.
- W2000268530 hasConcept C202751555 @default.
- W2000268530 hasConcept C203014093 @default.
- W2000268530 hasConcept C24998067 @default.
- W2000268530 hasConcept C2778690821 @default.
- W2000268530 hasConcept C40767141 @default.
- W2000268530 hasConcept C54355233 @default.
- W2000268530 hasConcept C55493867 @default.
- W2000268530 hasConcept C81885089 @default.
- W2000268530 hasConcept C86803240 @default.
- W2000268530 hasConcept C95444343 @default.
- W2000268530 hasConceptScore W2000268530C104317684 @default.
- W2000268530 hasConceptScore W2000268530C116073593 @default.
- W2000268530 hasConceptScore W2000268530C116569031 @default.
- W2000268530 hasConceptScore W2000268530C11988809 @default.
- W2000268530 hasConceptScore W2000268530C126037660 @default.
- W2000268530 hasConceptScore W2000268530C134018914 @default.
- W2000268530 hasConceptScore W2000268530C153911025 @default.
- W2000268530 hasConceptScore W2000268530C159654299 @default.
- W2000268530 hasConceptScore W2000268530C16613235 @default.
- W2000268530 hasConceptScore W2000268530C170493617 @default.
- W2000268530 hasConceptScore W2000268530C185592680 @default.
- W2000268530 hasConceptScore W2000268530C202751555 @default.
- W2000268530 hasConceptScore W2000268530C203014093 @default.
- W2000268530 hasConceptScore W2000268530C24998067 @default.
- W2000268530 hasConceptScore W2000268530C2778690821 @default.
- W2000268530 hasConceptScore W2000268530C40767141 @default.
- W2000268530 hasConceptScore W2000268530C54355233 @default.
- W2000268530 hasConceptScore W2000268530C55493867 @default.
- W2000268530 hasConceptScore W2000268530C81885089 @default.
- W2000268530 hasConceptScore W2000268530C86803240 @default.
- W2000268530 hasConceptScore W2000268530C95444343 @default.
- W2000268530 hasIssue "1" @default.
- W2000268530 hasLocation W20002685301 @default.
- W2000268530 hasLocation W20002685302 @default.
- W2000268530 hasOpenAccess W2000268530 @default.
- W2000268530 hasPrimaryLocation W20002685301 @default.
- W2000268530 hasRelatedWork W1966891309 @default.
- W2000268530 hasRelatedWork W2024090141 @default.
- W2000268530 hasRelatedWork W2059347528 @default.
- W2000268530 hasRelatedWork W2331538469 @default.
- W2000268530 hasRelatedWork W2360587303 @default.
- W2000268530 hasRelatedWork W2366823706 @default.
- W2000268530 hasRelatedWork W2368295268 @default.
- W2000268530 hasRelatedWork W2385139158 @default.
- W2000268530 hasRelatedWork W2409278405 @default.
- W2000268530 hasRelatedWork W2417380264 @default.
- W2000268530 hasVolume "147" @default.
- W2000268530 isParatext "false" @default.
- W2000268530 isRetracted "false" @default.
- W2000268530 magId "2000268530" @default.
- W2000268530 workType "article" @default.