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- W2000269595 abstract "Abstract An aqueous two-phase polymer system was used to isolate plasma membranes from a palpable mouse fibrosarcoma. The excised tumor tissue was washed with sterile saline and pushed through nylon screens of decreasing mesh size. This cell suspension was placed in Tris-buffered, isotonic sucrose plus MgSo4 and homogenized by nitrogen cavitation. A pellet was collected from the homogenate by low-speed centrifugation and was added to the aqueous two-phase polymer system. After several brief, low-speed centrifugations, the interfacial material between the polymer phases was collected. Data from enzyme and biochemical assays demonstrated that this fraction was plasma membrane. This method provided a high yield of the surface membrane in less than three hours." @default.
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- W2000269595 date "1974-01-01" @default.
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- W2000269595 title "Rapid Method for the Isolation of Plasma Membranes from a Murine Fibrosarcoma Using an Aqueous Two-Phase Polymer System" @default.
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- W2000269595 doi "https://doi.org/10.1080/00327487408061550" @default.
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