FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000274938> ?p ?o ?g. }

• W2000274938 endingPage "183" @default.
• W2000274938 startingPage "176" @default.
• W2000274938 abstract "Heat shock proteins have been shown to be secreted from a number of cell types. Necrotic cells release heat shock proteins in a passive manner, whereas we, and others, have shown that viable cells secrete Hsp70 and Hsp60 through an active mechanism involving lysosomal vesicles and lipid rafts. This release of Hsp70 and Hsp60 is regulated, for example by being increased by elevated temperature. This article outlines procedures, using Hsp70 as the example, to: ensure the status of cells (viable, apoptotic or necrotic); identify the heat shock protein secreted; and quantify the secreted protein. Hsp70 has previously been quantified by ELISA, but newer methods are now being adopted, such as BIAcore and bead-based assays for use by FACS. These methods have the advantages of being more sensitive and requiring less sample than ELISA. The BIAcore has the potential to analyse Hsp70 ligands and provide affinity constants. The FACS bead assay system can be used to run multiplex assays." @default.
• W2000274938 created "2016-06-24" @default.
• W2000274938 creator A5001798932 @default.
• W2000274938 creator A5021618423 @default.
• W2000274938 creator A5031427250 @default.
• W2000274938 creator A5032652630 @default.
• W2000274938 creator A5034216111 @default.
• W2000274938 creator A5073275494 @default.
• W2000274938 creator A5086670420 @default.
• W2000274938 date "2007-11-01" @default.
• W2000274938 modified "2023-09-30" @default.
• W2000274938 title "Measuring the secretion of heat shock proteins from cells" @default.
• W2000274938 cites W1483175931 @default.
• W2000274938 cites W1507205945 @default.
• W2000274938 cites W1964567745 @default.
• W2000274938 cites W1971756409 @default.
• W2000274938 cites W1988687954 @default.
• W2000274938 cites W1992038850 @default.
• W2000274938 cites W2001524048 @default.
• W2000274938 cites W2005053745 @default.
• W2000274938 cites W2006104618 @default.
• W2000274938 cites W2008739531 @default.
• W2000274938 cites W2009910874 @default.
• W2000274938 cites W2011641581 @default.
• W2000274938 cites W2015535625 @default.
• W2000274938 cites W2016145324 @default.
• W2000274938 cites W2016223924 @default.
• W2000274938 cites W2017047609 @default.
• W2000274938 cites W2020126424 @default.
• W2000274938 cites W2027442935 @default.
• W2000274938 cites W2037404930 @default.
• W2000274938 cites W2049980156 @default.
• W2000274938 cites W2051920652 @default.
• W2000274938 cites W2056133274 @default.
• W2000274938 cites W2067414008 @default.
• W2000274938 cites W2071923005 @default.
• W2000274938 cites W2076314315 @default.
• W2000274938 cites W2077926643 @default.
• W2000274938 cites W2091021277 @default.
• W2000274938 cites W2097070639 @default.
• W2000274938 cites W2097252194 @default.
• W2000274938 cites W2130968423 @default.
• W2000274938 cites W2145736817 @default.
• W2000274938 cites W2155518512 @default.
• W2000274938 cites W2163443542 @default.
• W2000274938 cites W2169671282 @default.
• W2000274938 cites W4244070538 @default.
• W2000274938 cites W59259273 @default.
• W2000274938 doi "https://doi.org/10.1016/j.ymeth.2007.06.011" @default.
• W2000274938 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17920513" @default.
• W2000274938 hasPublicationYear "2007" @default.
• W2000274938 type Work @default.
• W2000274938 sameAs 2000274938 @default.
• W2000274938 citedByCount "38" @default.
• W2000274938 countsByYear W20002749382012 @default.
• W2000274938 countsByYear W20002749382013 @default.
• W2000274938 countsByYear W20002749382014 @default.
• W2000274938 countsByYear W20002749382015 @default.
• W2000274938 countsByYear W20002749382016 @default.
• W2000274938 countsByYear W20002749382017 @default.
• W2000274938 countsByYear W20002749382019 @default.
• W2000274938 countsByYear W20002749382020 @default.
• W2000274938 countsByYear W20002749382021 @default.
• W2000274938 countsByYear W20002749382022 @default.
• W2000274938 crossrefType "journal-article" @default.
• W2000274938 hasAuthorship W2000274938A5001798932 @default.
• W2000274938 hasAuthorship W2000274938A5021618423 @default.
• W2000274938 hasAuthorship W2000274938A5031427250 @default.
• W2000274938 hasAuthorship W2000274938A5032652630 @default.
• W2000274938 hasAuthorship W2000274938A5034216111 @default.
• W2000274938 hasAuthorship W2000274938A5073275494 @default.
• W2000274938 hasAuthorship W2000274938A5086670420 @default.
• W2000274938 hasConcept C104317684 @default.
• W2000274938 hasConcept C126322002 @default.
• W2000274938 hasConcept C137984847 @default.
• W2000274938 hasConcept C145059251 @default.
• W2000274938 hasConcept C146621620 @default.
• W2000274938 hasConcept C153911025 @default.
• W2000274938 hasConcept C185592680 @default.
• W2000274938 hasConcept C20518536 @default.
• W2000274938 hasConcept C205260736 @default.
• W2000274938 hasConcept C2781300812 @default.
• W2000274938 hasConcept C49039625 @default.
• W2000274938 hasConcept C55493867 @default.
• W2000274938 hasConcept C68991219 @default.
• W2000274938 hasConcept C71924100 @default.
• W2000274938 hasConcept C86803240 @default.
• W2000274938 hasConcept C95444343 @default.
• W2000274938 hasConceptScore W2000274938C104317684 @default.
• W2000274938 hasConceptScore W2000274938C126322002 @default.
• W2000274938 hasConceptScore W2000274938C137984847 @default.
• W2000274938 hasConceptScore W2000274938C145059251 @default.
• W2000274938 hasConceptScore W2000274938C146621620 @default.
• W2000274938 hasConceptScore W2000274938C153911025 @default.
• W2000274938 hasConceptScore W2000274938C185592680 @default.
• W2000274938 hasConceptScore W2000274938C20518536 @default.
• W2000274938 hasConceptScore W2000274938C205260736 @default.
• W2000274938 hasConceptScore W2000274938C2781300812 @default.

• next