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- W2000277415 abstract "A native milk fat globule membrane (MFGM) isolate obtained from raw milk was assessed for its anticarcinogenic capacity using a colon cancer cell line (HT-29). To prevent microbial contamination and eliminate the presence of lipopolysaccharide (LPS) in the milk used for MFGM isolation, the milk was obtained from the mammary glands of cows using a catheter. Cell proliferation assays demonstrated a reduction of exponentially growing cancer cells of up to 53%, expressed as DNA synthesis (BrdU test), after 72 h stimulation with 100 μg of MFGM protein per mL. Using a similar MFGM concentration, the sulforhodamine B assay resulted in 57% reduction of cell density after 48 h incubation. This bioactivity was comparable to that of known anticancer drugs, 0.1 mM melphalan and 20 μM C2-ceramide, which achieved a cell division reduction of 25 and 40%, respectively, under the same experimental conditions. The toxic effect of the MFGM extracts on HT-29 cells was confirmed by the significant reduction in lactate dehydrogenase enzyme (LDH) by the residual viable cells. An increase of caspase-3 activity (up to 26%) led to the conclusion that MFGM has an apoptotic effect on HT-29 cancer cells." @default.
- W2000277415 created "2016-06-24" @default.
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- W2000277415 creator A5008657302 @default.
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- W2000277415 date "2013-01-01" @default.
- W2000277415 modified "2023-10-14" @default.
- W2000277415 title "Milk fat globule membrane isolate induces apoptosis in HT-29 human colon cancer cells" @default.
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- W2000277415 doi "https://doi.org/10.1039/c2fo30189j" @default.
- W2000277415 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23070113" @default.
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