FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000277875> ?p ?o ?g. }

• W2000277875 endingPage "553" @default.
• W2000277875 startingPage "536" @default.
• W2000277875 abstract "Bacteriorhodopsin (bR) is the simplest known light driven proton pump and has been heavily studied using structural methods: eighty four X-ray diffraction, six electron diffraction and three NMR structures of bR are deposited within the protein data bank. Twenty one X-ray structures report light induced structural changes and changes induced by mutation, changes in pH, thermal annealing or X-ray induced photo-reduction have also been examined.We argue that light-induced structural changes that are replicated across several studies by independent research groups are those most likely to represent what is happening in reality. We present both internal distance matrix analyses that sort deposited bR structures into hierarchal trees, and difference Fourier analysis of deposited X-ray diffraction data.An internal distance matrix analysis separates most wild-type bR structures according to their different crystal forms, indicating how the protein's structure is influenced by crystallization conditions. A similar analysis clusters eleven studies of illuminated bR crystals as one branch of a hierarchal tree with reproducible movements of the extracellular portion of helix C towards helix G, and of the cytoplasmic portion of helix F away from helices A, B and G. All crystallographic data deposited for illuminated crystals show negative difference density on a water molecule (Wat402) that forms H-bonds to the retinal Schiff Base and two aspartate residues (Asp85, Asp212) in the bR resting state. Other recurring difference density features indicated reproducible side-chain, backbone and water molecule displacements. X-ray induced radiation damage also disorders Wat402 but acts via cleaving the head-groups of Asp85 and Asp212.A remarkable level of agreement exists when deposited structures and crystallographic observations are viewed as a whole. From this agreement a unified picture of the structural mechanism of light-induced proton pumping by bR emerges. This article is part of a Special Issue entitled Structural biochemistry and biophysics of membrane proteins." @default.
• W2000277875 created "2016-06-24" @default.
• W2000277875 creator A5011809219 @default.
• W2000277875 creator A5017316562 @default.
• W2000277875 creator A5043617948 @default.
• W2000277875 creator A5044448446 @default.
• W2000277875 date "2015-03-01" @default.
• W2000277875 modified "2023-09-29" @default.
• W2000277875 title "Bacteriorhodopsin: Would the real structural intermediates please stand up?" @default.
• W2000277875 cites W1508315241 @default.
• W2000277875 cites W1578292667 @default.
• W2000277875 cites W1586792701 @default.
• W2000277875 cites W1594929565 @default.
• W2000277875 cites W1620064517 @default.
• W2000277875 cites W162442128 @default.
• W2000277875 cites W1785940294 @default.
• W2000277875 cites W1825353903 @default.
• W2000277875 cites W1832700322 @default.
• W2000277875 cites W1907544268 @default.
• W2000277875 cites W1942533571 @default.
• W2000277875 cites W1963480398 @default.
• W2000277875 cites W1968458945 @default.
• W2000277875 cites W1968960649 @default.
• W2000277875 cites W1970538439 @default.
• W2000277875 cites W1972014516 @default.
• W2000277875 cites W1972563656 @default.
• W2000277875 cites W1973881708 @default.
• W2000277875 cites W1974224535 @default.
• W2000277875 cites W1975934353 @default.
• W2000277875 cites W1977464493 @default.
• W2000277875 cites W1977569471 @default.
• W2000277875 cites W1978886142 @default.
• W2000277875 cites W1979565363 @default.
• W2000277875 cites W1984660994 @default.
• W2000277875 cites W1986789979 @default.
• W2000277875 cites W1987418575 @default.
• W2000277875 cites W1988097700 @default.
• W2000277875 cites W1990798950 @default.
• W2000277875 cites W1994152613 @default.
• W2000277875 cites W1997263768 @default.
• W2000277875 cites W1999768562 @default.
• W2000277875 cites W2004929882 @default.
• W2000277875 cites W2009038821 @default.
• W2000277875 cites W2010622172 @default.
• W2000277875 cites W2012670872 @default.
• W2000277875 cites W2014445498 @default.
• W2000277875 cites W2016066619 @default.
• W2000277875 cites W2016237935 @default.
• W2000277875 cites W2017393510 @default.
• W2000277875 cites W2017668510 @default.
• W2000277875 cites W2019306938 @default.
• W2000277875 cites W2020465369 @default.
• W2000277875 cites W2021661744 @default.
• W2000277875 cites W2024741993 @default.
• W2000277875 cites W2030175542 @default.
• W2000277875 cites W2033783558 @default.
• W2000277875 cites W2034397295 @default.
• W2000277875 cites W2036862423 @default.
• W2000277875 cites W2037779370 @default.
• W2000277875 cites W2041256732 @default.
• W2000277875 cites W2045130774 @default.
• W2000277875 cites W2048695489 @default.
• W2000277875 cites W2050292589 @default.
• W2000277875 cites W2050421068 @default.
• W2000277875 cites W2051068302 @default.
• W2000277875 cites W2051956840 @default.
• W2000277875 cites W2054091316 @default.
• W2000277875 cites W2054288728 @default.
• W2000277875 cites W2055425903 @default.
• W2000277875 cites W2056316643 @default.
• W2000277875 cites W2056641846 @default.
• W2000277875 cites W2058209954 @default.
• W2000277875 cites W2059043635 @default.
• W2000277875 cites W2062021082 @default.
• W2000277875 cites W2062930912 @default.
• W2000277875 cites W2064497637 @default.
• W2000277875 cites W2067079920 @default.
• W2000277875 cites W2067841258 @default.
• W2000277875 cites W2069238522 @default.
• W2000277875 cites W2069339217 @default.
• W2000277875 cites W2069696932 @default.
• W2000277875 cites W2072405301 @default.
• W2000277875 cites W2072962112 @default.
• W2000277875 cites W2073272276 @default.
• W2000277875 cites W2074062743 @default.
• W2000277875 cites W2075592322 @default.
• W2000277875 cites W2076057575 @default.
• W2000277875 cites W2077431803 @default.
• W2000277875 cites W2080231167 @default.
• W2000277875 cites W2081252646 @default.
• W2000277875 cites W2081417841 @default.
• W2000277875 cites W2083277777 @default.
• W2000277875 cites W2085191928 @default.
• W2000277875 cites W2093562743 @default.
• W2000277875 cites W2104477830 @default.
• W2000277875 cites W2104675030 @default.
• W2000277875 cites W2108455349 @default.
• W2000277875 cites W2110810808 @default.

• next