FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000278895> ?p ?o ?g. }

• W2000278895 endingPage "1104" @default.
• W2000278895 startingPage "1094" @default.
• W2000278895 abstract "Macrophages are involved in immediate hypersensitivity reactions by their ability to release leukotrienes involved in the symptomatology of allergy. To date it is unknown whether this ability to secrete leukotrienes has been favoured by modifications, occurring during the sensitization phase, of the enzymes involved in leukotriene metabolism.We used ovalbumin-sensitized rats to study the expression of cytosolic phospholipase A2 (cPLA2), 5-lipoxygenase (5-LO) and 5-lipoxygenase-activating protein (FLAP) in peritoneal macrophages during active sensitization. We compared basal and challenged (PMA, A23187 and allergen) arachidonic acid (AA) metabolism of macrophages from control (cPM) and sensitized (sPM) rats. Then we tested, in cultured cPM, whether IL-4, the predominant cytokine of sensitization process, could reproduce the enzymatic modifications occurring in macrophages during sensitization.cPLA2, 5-LO and FLAP expression was assessed by Western blotting. The arachidonic acid (AA) metabolism study was performed after incorporation of tritiated AA in macrophages and analysis of secreted tritiated eicosanoids.Ovalbumin-sensitization of rats increased cPLA2, 5-LO and FLAP expression in peritoneal macrophages. These increased expressions were not paralleled by modifications of basal and PMA- or A23187-stimulated AA metabolism of sPM. However, when macrophages encountered the specific allergen for a second time, sPM secreted higher levels of leukotrienes than cPM. IL-4 induced FLAP expression in cPM but had no effect on cPLA2 and 5-LO expression.Active sensitization of rats induces an increase, in peritoneal macrophages, of the enzymes involved in leukotriene metabolism. The increased leukotriene secretion of sPM in response to ovalbumin challenge may be favoured by this increased expression of cPLA2, 5-LO and FLAP that, however, is not able to lead to modifications of macrophage AA metabolism in any circumstance. Our results also suggest that IL-4 is not the major element originating the enzymatic modification induced by sensitization in peritoneal macrophages." @default.
• W2000278895 created "2016-06-24" @default.
• W2000278895 creator A5000592346 @default.
• W2000278895 creator A5017074730 @default.
• W2000278895 creator A5021896227 @default.
• W2000278895 creator A5024474125 @default.
• W2000278895 creator A5027844665 @default.
• W2000278895 creator A5048084277 @default.
• W2000278895 creator A5058603510 @default.
• W2000278895 creator A5068459453 @default.
• W2000278895 date "2001-07-01" @default.
• W2000278895 modified "2023-10-18" @default.
• W2000278895 title "Increased expression of cytosolic phospholipase A2, 5-lipoxygenase and 5-lipoxygenase-activating protein in rat peritoneal macrophages during ovalbumin-induced sensitization" @default.
• W2000278895 cites W1491336114 @default.
• W2000278895 cites W1496063181 @default.
• W2000278895 cites W1553604057 @default.
• W2000278895 cites W1601600505 @default.
• W2000278895 cites W1969912242 @default.
• W2000278895 cites W1980832959 @default.
• W2000278895 cites W1982407164 @default.
• W2000278895 cites W1982523785 @default.
• W2000278895 cites W1985572161 @default.
• W2000278895 cites W1994730705 @default.
• W2000278895 cites W1997531619 @default.
• W2000278895 cites W2006982389 @default.
• W2000278895 cites W2014768234 @default.
• W2000278895 cites W2018522670 @default.
• W2000278895 cites W2019353213 @default.
• W2000278895 cites W2021330009 @default.
• W2000278895 cites W2024976994 @default.
• W2000278895 cites W2025901050 @default.
• W2000278895 cites W2027145809 @default.
• W2000278895 cites W2028274305 @default.
• W2000278895 cites W2034146535 @default.
• W2000278895 cites W2038575244 @default.
• W2000278895 cites W2044673785 @default.
• W2000278895 cites W2051271931 @default.
• W2000278895 cites W2051958752 @default.
• W2000278895 cites W2057345518 @default.
• W2000278895 cites W2060763719 @default.
• W2000278895 cites W2063700992 @default.
• W2000278895 cites W2075317418 @default.
• W2000278895 cites W2078146109 @default.
• W2000278895 cites W2078571876 @default.
• W2000278895 cites W2081599952 @default.
• W2000278895 cites W2086547881 @default.
• W2000278895 cites W2090806469 @default.
• W2000278895 cites W2093541746 @default.
• W2000278895 cites W2094300672 @default.
• W2000278895 cites W2096381182 @default.
• W2000278895 cites W2121737195 @default.
• W2000278895 cites W2123733931 @default.
• W2000278895 cites W2124566107 @default.
• W2000278895 cites W2125805192 @default.
• W2000278895 cites W2140901513 @default.
• W2000278895 cites W2146388445 @default.
• W2000278895 cites W2150521669 @default.
• W2000278895 doi "https://doi.org/10.1046/j.1365-2222.2001.01121.x" @default.
• W2000278895 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11468001" @default.
• W2000278895 hasPublicationYear "2001" @default.
• W2000278895 type Work @default.
• W2000278895 sameAs 2000278895 @default.
• W2000278895 citedByCount "5" @default.
• W2000278895 countsByYear W20002788952013 @default.
• W2000278895 crossrefType "journal-article" @default.
• W2000278895 hasAuthorship W2000278895A5000592346 @default.
• W2000278895 hasAuthorship W2000278895A5017074730 @default.
• W2000278895 hasAuthorship W2000278895A5021896227 @default.
• W2000278895 hasAuthorship W2000278895A5024474125 @default.
• W2000278895 hasAuthorship W2000278895A5027844665 @default.
• W2000278895 hasAuthorship W2000278895A5048084277 @default.
• W2000278895 hasAuthorship W2000278895A5058603510 @default.
• W2000278895 hasAuthorship W2000278895A5068459453 @default.
• W2000278895 hasConcept C134018914 @default.
• W2000278895 hasConcept C141105273 @default.
• W2000278895 hasConcept C147483822 @default.
• W2000278895 hasConcept C159654299 @default.
• W2000278895 hasConcept C181199279 @default.
• W2000278895 hasConcept C185592680 @default.
• W2000278895 hasConcept C203014093 @default.
• W2000278895 hasConcept C2776042228 @default.
• W2000278895 hasConcept C2776217527 @default.
• W2000278895 hasConcept C2776452501 @default.
• W2000278895 hasConcept C2776551241 @default.
• W2000278895 hasConcept C2776685179 @default.
• W2000278895 hasConcept C2776888444 @default.
• W2000278895 hasConcept C2776914184 @default.
• W2000278895 hasConcept C2776946954 @default.
• W2000278895 hasConcept C2778078955 @default.
• W2000278895 hasConcept C2779543280 @default.
• W2000278895 hasConcept C2780857362 @default.
• W2000278895 hasConcept C49039625 @default.
• W2000278895 hasConcept C55493867 @default.
• W2000278895 hasConcept C86803240 @default.
• W2000278895 hasConcept C90342290 @default.
• W2000278895 hasConceptScore W2000278895C134018914 @default.
• W2000278895 hasConceptScore W2000278895C141105273 @default.
• W2000278895 hasConceptScore W2000278895C147483822 @default.

• next