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• W2000283985 abstract "Laboratory experiments using stable Cd isotopes (110Cd and 112Cd) were conducted to separately and simultaneously characterize Cd accumulation in different tissues of Pacific oysters (Crassostrea gigas) via the (i) trophic and (ii) direct pathways. For this, we exposed juvenile oysters to 110Cd-spiked seawater (110Cd: 2 μg l−1; constant level) and 112Cd-spiked food (Thalassiossera weissflogii, 112Cd: 2 μg l−1 in 35×103 cells/oyster/L) in four experimental treatment groups, each containing 6 oysters, for 21 days with constant trophic feeding. These Cd contamination levels were ∼10 times lower than those typically used in experimental accumulation studies. Three oysters per treatment group were dissected every 7 days with separate sampling of the gills, digestive gland and the rest of the body. Metallothioneins were analysed in the digestive gland and gills. Cadmium concentrations and isotope ratios were measured in water (daily) and tissues (weekly) by GF-AAS and ICP-MS. The observed time-dependant evolution in Cd concentrations and 110Cd/114Cd and 112Cd/114Cd isotope ratios clearly revealed the bio-accumulation short-term kinetics and pathways of Cd contamination in the different tissues. Under the experimental conditions, significantly changed isotope ratios in gills and the digestive gland of oysters suggested rapid and efficient contamination by 110Cd derived from direct exposure followed by internal Cd transfer between organs. Trophic contamination became measurable after 14 days of exposure corresponding to a trophic transfer rate of 1%. Constant metallothionein levels during the experiment suggested that the initially present metallothionein levels were sufficient to deal with the experimental Cd exposure." @default.
• W2000283985 created "2016-06-24" @default.
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• W2000283985 date "2011-05-01" @default.
• W2000283985 modified "2023-10-06" @default.
• W2000283985 title "Tracing cadmium contamination kinetics and pathways in oysters (Crassostrea gigas) by multiple stable Cd isotope spike experiments" @default.
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• W2000283985 doi "https://doi.org/10.1016/j.ecoenv.2010.10.020" @default.
• W2000283985 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21035189" @default.
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