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- W2000287432 abstract "A precise method is presented for determination of residual spiramycin (SP) in chicken eggs and tissues by high-performance liquid chromatography (HPLC). The sample preparation was performed by homogenizing with a mixture of acetonitrile and n-hexane (5:4, v/v) to minimize the fat amount followed by ultra-filtration using a MolCutII®. The extracts containing SP were free from interfering compounds when examined by the normal-phase HPLC using a LiChrosorb® NH2 column and a mobile phase of acetonitrile–water (85:15, v/v) with a photo-diode array detector. The average recoveries from spiked SP (0.1, 0.5 and 1.0 ppm) were in excess of 89.0% with coefficients of variation between 1.4 and 2.4%. The limit of detection was 0.1 ppm." @default.
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- W2000287432 date "1999-06-01" @default.
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- W2000287432 title "Normal-phase high-performance liquid chromatographic determination of spiramycin in eggs and chicken" @default.
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- W2000287432 doi "https://doi.org/10.1016/s0039-9140(99)00043-0" @default.
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