FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000288364> ?p ?o ?g. }

• W2000288364 endingPage "12" @default.
• W2000288364 startingPage "1" @default.
• W2000288364 abstract "Bone sialoprotein (BSP) is a noncollagenous protein of the extracellular matrix in mineralized connective tissues that has been implicated in the nucleation of hydroxyapatite. Forskolin (FSK), an activator of adenylate cyclase, increased the intracellular cAMP level, which stimulates the proliferation and differentiation of osteoblasts. Fibroblast growth factor 2 (FGF2) is a potent mitogen in many cell types, including osteoblasts. In human prostate cancer DU145 cells, FSK (1 μM) and FGF2 (10 ng/ml) increased BSP and Runx2 mRNA and protein levels at 3 and 12 h, respectively. Transient transfection analyses were performed using chimeric constructs of the human BSP gene promoter linked to a luciferase reporter gene. Treatment of DU145 cells with FSK (1 μM) and FGF2 (10 ng/ml) increased the luciferase activities of constructs between –60LUC to –927LUC and –108LUC to –927LUC, including the human BSP gene promoter. Effects of FSK and FGF2 abrogated in constructs included 2 bp mutations in the two cAMP response elements (CRE1 and CRE2). Luciferase activities induced by FSK and FGF2 were blocked by protein kinase A and tyrosine kinase inhibitors. Gel mobility shift analyses showed that FSK and FGF2 increased the binding of CRE1 and CRE2. CRE1–protein complexes were supershifted by phospho-CREB1 and c-Fos antibodies, and disrupted by CREB1, c-Jun, JunD, Fra2, p300, Runx2, Dlx5 and Smad1 antibodies. CRE2–protein complexes were disrupted by CREB1, phospho-CREB1, c-Fos, c-Jun, JunD, Fra2, p300, Runx2, Dlx5 and Smad1 antibodies. These studies demonstrate that FSK and FGF2 stimulate BSP transcription in DU145 human prostate cancer cells by targeting the CRE1 and CRE2 elements in the human BSP gene promoter." @default.
• W2000288364 created "2016-06-24" @default.
• W2000288364 creator A5004152602 @default.
• W2000288364 creator A5007332536 @default.
• W2000288364 creator A5007949831 @default.
• W2000288364 creator A5018263002 @default.
• W2000288364 creator A5021659645 @default.
• W2000288364 creator A5024541563 @default.
• W2000288364 creator A5035520440 @default.
• W2000288364 creator A5040743786 @default.
• W2000288364 creator A5041893900 @default.
• W2000288364 creator A5067100305 @default.
• W2000288364 creator A5072365724 @default.
• W2000288364 creator A5073154054 @default.
• W2000288364 date "2011-01-01" @default.
• W2000288364 modified "2023-10-13" @default.
• W2000288364 title "cAMP and fibroblast growth factor 2 regulate bone sialoprotein gene expression in human prostate cancer cells" @default.
• W2000288364 cites W1450960572 @default.
• W2000288364 cites W1511542029 @default.
• W2000288364 cites W1517339234 @default.
• W2000288364 cites W1663371441 @default.
• W2000288364 cites W1757636880 @default.
• W2000288364 cites W1885709862 @default.
• W2000288364 cites W1970874606 @default.
• W2000288364 cites W1980014652 @default.
• W2000288364 cites W1984362855 @default.
• W2000288364 cites W1993243592 @default.
• W2000288364 cites W1998352109 @default.
• W2000288364 cites W2008073344 @default.
• W2000288364 cites W2009291798 @default.
• W2000288364 cites W2016091065 @default.
• W2000288364 cites W2022920312 @default.
• W2000288364 cites W2026888556 @default.
• W2000288364 cites W2027448452 @default.
• W2000288364 cites W2037612886 @default.
• W2000288364 cites W2038147666 @default.
• W2000288364 cites W2040195944 @default.
• W2000288364 cites W2045171889 @default.
• W2000288364 cites W2047256275 @default.
• W2000288364 cites W2048831133 @default.
• W2000288364 cites W2051849197 @default.
• W2000288364 cites W2053926227 @default.
• W2000288364 cites W2059085904 @default.
• W2000288364 cites W2065718179 @default.
• W2000288364 cites W2066036961 @default.
• W2000288364 cites W2067077186 @default.
• W2000288364 cites W2071680116 @default.
• W2000288364 cites W2077228584 @default.
• W2000288364 cites W2082625784 @default.
• W2000288364 cites W2083186952 @default.
• W2000288364 cites W2086905843 @default.
• W2000288364 cites W2091910695 @default.
• W2000288364 cites W2109218792 @default.
• W2000288364 cites W2116903136 @default.
• W2000288364 cites W2122374296 @default.
• W2000288364 cites W2123927174 @default.
• W2000288364 cites W2124843577 @default.
• W2000288364 cites W2126482839 @default.
• W2000288364 cites W2138354687 @default.
• W2000288364 cites W2140099557 @default.
• W2000288364 cites W2163218047 @default.
• W2000288364 cites W2167091901 @default.
• W2000288364 cites W2168500016 @default.
• W2000288364 cites W2169640925 @default.
• W2000288364 cites W2171561617 @default.
• W2000288364 cites W2180410797 @default.
• W2000288364 cites W2403847737 @default.
• W2000288364 cites W4211200865 @default.
• W2000288364 doi "https://doi.org/10.1016/j.gene.2010.09.009" @default.
• W2000288364 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20965237" @default.
• W2000288364 hasPublicationYear "2011" @default.
• W2000288364 type Work @default.
• W2000288364 sameAs 2000288364 @default.
• W2000288364 citedByCount "14" @default.
• W2000288364 countsByYear W20002883642012 @default.
• W2000288364 countsByYear W20002883642013 @default.
• W2000288364 countsByYear W20002883642014 @default.
• W2000288364 countsByYear W20002883642017 @default.
• W2000288364 countsByYear W20002883642022 @default.
• W2000288364 countsByYear W20002883642023 @default.
• W2000288364 crossrefType "journal-article" @default.
• W2000288364 hasAuthorship W2000288364A5004152602 @default.
• W2000288364 hasAuthorship W2000288364A5007332536 @default.
• W2000288364 hasAuthorship W2000288364A5007949831 @default.
• W2000288364 hasAuthorship W2000288364A5018263002 @default.
• W2000288364 hasAuthorship W2000288364A5021659645 @default.
• W2000288364 hasAuthorship W2000288364A5024541563 @default.
• W2000288364 hasAuthorship W2000288364A5035520440 @default.
• W2000288364 hasAuthorship W2000288364A5040743786 @default.
• W2000288364 hasAuthorship W2000288364A5041893900 @default.
• W2000288364 hasAuthorship W2000288364A5067100305 @default.
• W2000288364 hasAuthorship W2000288364A5072365724 @default.
• W2000288364 hasAuthorship W2000288364A5073154054 @default.
• W2000288364 hasConcept C104317684 @default.
• W2000288364 hasConcept C121608353 @default.
• W2000288364 hasConcept C126322002 @default.
• W2000288364 hasConcept C134018914 @default.
• W2000288364 hasConcept C153911025 @default.

• next