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- W2000289929 abstract "To assess the viability and function of human sperm in electrolyte-free cold preservation.Prospective comparative study.Andrology laboratory of our hospital.Ten semen samples obtained from patients attending our infertility clinic.Ejaculated sperm were washed using the electrolyte-free Percoll gradient and were then preserved in 0.33 M glucose solution, 0.16 M NaCl solution, 0.16 M KCl solution at 4 degrees C for 4 weeks. As a control, TEST (TES and Tris) yolk buffer (TYB) was added to the ejaculated semen and preserved at 4 degrees C.Sperm tail morphology, motility, viability (eosin-Y stain), and the concentration of adenosine triphosphate (ATP) were analyzed.The number of sperm with normal tail form and the motility of sperm preserved in glucose solution (electrolyte-free cold preservation) were significantly (P < 0.01) higher for 4 weeks than those of sperm preserved in the other three media. The sperm viability in glucose solution was 75.5%, 65.4%, and 51.3%, after 1, 2, and 4 weeks, respectively. The ATP concentration after 1, 2, and 4 weeks remained 64.2%, 53.0%, and 4.3% of the prestorage value, respectively, in the sperm stored in glucose solution.The morphology, motility, viability, and ATP concentration of sperm in electrolyte-free cold preservation were substantially better than those in NaCl solution, KCl solution, or TYB for 2 weeks." @default.
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- W2000289929 date "1998-01-01" @default.
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- W2000289929 title "Viability and function of human sperm in electrolyte-free cold preservation" @default.
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- W2000289929 doi "https://doi.org/10.1016/s0015-0282(97)00439-1" @default.
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