FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000290629> ?p ?o ?g. }

• W2000290629 endingPage "010" @default.
• W2000290629 startingPage "005" @default.
• W2000290629 abstract "A method for improved refolding and purification of E. coli derived human Interferon -? (rhIFN ?2b) from inclusion bodies as a Staphylokinase (SAK) fusion protein is described. Such a fusion protein did not require the supplementation of rare codons for expression and was found to be stable at 37?C. The optimal conditions of refolding involved the use of a mild denaturating agent without the need for any other agents to prevent aggregation. The SAKrhIFN ?2b fusion protein was successfully purified using two steps of purification and was cleaved using enterokinase into two fragments namely SAK and IFN. Both the proteins were found to be biologically active showing proper folding of both the fusion partners. The cleaved IFN showed similar retention time on RP-HPLC as the bacterial derived untagged purified IFN as well as similar molecular weight on Agilent 2100 Bioanalyzer indicating the right processing of the IFN after enterokinase cleavage. The expression levels of SAK-IFN were found to be two folds higher than that observed with untagged IFN under similar experimental conditions." @default.
• W2000290629 created "2016-06-24" @default.
• W2000290629 creator A5053704847 @default.
• W2000290629 creator A5062614455 @default.
• W2000290629 creator A5070552821 @default.
• W2000290629 creator A5071008348 @default.
• W2000290629 creator A5090741454 @default.
• W2000290629 date "2009-01-01" @default.
• W2000290629 modified "2023-09-27" @default.
• W2000290629 title "Expression and Purification of SAK-fused Human Interferon Alpha in Escherichia coli" @default.
• W2000290629 cites W1515288612 @default.
• W2000290629 cites W1978955012 @default.
• W2000290629 cites W1988212558 @default.
• W2000290629 cites W2022241614 @default.
• W2000290629 cites W2038428454 @default.
• W2000290629 cites W2049474034 @default.
• W2000290629 cites W2051291153 @default.
• W2000290629 cites W2052242127 @default.
• W2000290629 cites W2052863486 @default.
• W2000290629 cites W2058558811 @default.
• W2000290629 cites W2060423377 @default.
• W2000290629 cites W2061785757 @default.
• W2000290629 cites W2065815211 @default.
• W2000290629 cites W2071235304 @default.
• W2000290629 cites W2091685461 @default.
• W2000290629 cites W2092351519 @default.
• W2000290629 cites W2131032184 @default.
• W2000290629 cites W2134857592 @default.
• W2000290629 cites W2141227683 @default.
• W2000290629 cites W2143782771 @default.
• W2000290629 cites W2162629268 @default.
• W2000290629 cites W2177943720 @default.
• W2000290629 cites W2266915557 @default.
• W2000290629 cites W2333452860 @default.
• W2000290629 cites W26295571 @default.
• W2000290629 cites W3020839822 @default.
• W2000290629 doi "https://doi.org/10.4172/1948-5948.1000002" @default.
• W2000290629 hasPublicationYear "2009" @default.
• W2000290629 type Work @default.
• W2000290629 sameAs 2000290629 @default.
• W2000290629 citedByCount "6" @default.
• W2000290629 countsByYear W20002906292013 @default.
• W2000290629 countsByYear W20002906292014 @default.
• W2000290629 countsByYear W20002906292016 @default.
• W2000290629 countsByYear W20002906292017 @default.
• W2000290629 countsByYear W20002906292022 @default.
• W2000290629 crossrefType "journal-article" @default.
• W2000290629 hasAuthorship W2000290629A5053704847 @default.
• W2000290629 hasAuthorship W2000290629A5062614455 @default.
• W2000290629 hasAuthorship W2000290629A5070552821 @default.
• W2000290629 hasAuthorship W2000290629A5071008348 @default.
• W2000290629 hasAuthorship W2000290629A5090741454 @default.
• W2000290629 hasBestOaLocation W20002906291 @default.
• W2000290629 hasConcept C104317684 @default.
• W2000290629 hasConcept C105795698 @default.
• W2000290629 hasConcept C159047783 @default.
• W2000290629 hasConcept C171606756 @default.
• W2000290629 hasConcept C2776178377 @default.
• W2000290629 hasConcept C2909179924 @default.
• W2000290629 hasConcept C33923547 @default.
• W2000290629 hasConcept C49453240 @default.
• W2000290629 hasConcept C547475151 @default.
• W2000290629 hasConcept C55493867 @default.
• W2000290629 hasConcept C64943373 @default.
• W2000290629 hasConcept C70721500 @default.
• W2000290629 hasConcept C86803240 @default.
• W2000290629 hasConcept C89423630 @default.
• W2000290629 hasConceptScore W2000290629C104317684 @default.
• W2000290629 hasConceptScore W2000290629C105795698 @default.
• W2000290629 hasConceptScore W2000290629C159047783 @default.
• W2000290629 hasConceptScore W2000290629C171606756 @default.
• W2000290629 hasConceptScore W2000290629C2776178377 @default.
• W2000290629 hasConceptScore W2000290629C2909179924 @default.
• W2000290629 hasConceptScore W2000290629C33923547 @default.
• W2000290629 hasConceptScore W2000290629C49453240 @default.
• W2000290629 hasConceptScore W2000290629C547475151 @default.
• W2000290629 hasConceptScore W2000290629C55493867 @default.
• W2000290629 hasConceptScore W2000290629C64943373 @default.
• W2000290629 hasConceptScore W2000290629C70721500 @default.
• W2000290629 hasConceptScore W2000290629C86803240 @default.
• W2000290629 hasConceptScore W2000290629C89423630 @default.
• W2000290629 hasIssue "01" @default.
• W2000290629 hasLocation W20002906291 @default.
• W2000290629 hasOpenAccess W2000290629 @default.
• W2000290629 hasPrimaryLocation W20002906291 @default.
• W2000290629 hasRelatedWork W1496537660 @default.
• W2000290629 hasRelatedWork W1997868973 @default.
• W2000290629 hasRelatedWork W2005213780 @default.
• W2000290629 hasRelatedWork W2023856728 @default.
• W2000290629 hasRelatedWork W2046045290 @default.
• W2000290629 hasRelatedWork W2093200039 @default.
• W2000290629 hasRelatedWork W2415690445 @default.
• W2000290629 hasRelatedWork W2419515456 @default.
• W2000290629 hasRelatedWork W2470167225 @default.
• W2000290629 hasRelatedWork W4310621935 @default.
• W2000290629 hasVolume "01" @default.
• W2000290629 isParatext "false" @default.
• W2000290629 isRetracted "false" @default.

• next