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• W2000290686 abstract "The transmembrane aspartate receptor of E. coli and S. typhimurium mediates cellular chemotaxis toward aspartate by regulating the activity of the cytoplasmic histidine kinase, CheA. Ligand binding results in transduction of a conformational signal through the membrane to the cytoplasmic domain where both kinase regulation and adaptation occur. Of particular interest is the linker region, E213 to Q258, which connects and transduces the conformational signal between the cytoplasmic end of the transmembrane signaling helix (α4/TM2) and the major methylation helix of the cytoplasmic domain (α6). This linker is crucial for stable folding and function of the homodimeric receptor. The present study uses cysteine and disulfide scanning mutagenesis to investigate the secondary structure and packing surfaces within the linker region. Chemical reactivity assays reveal that the linker consists of three distinct subdomains: two α-helices termed α4 and α5 and, between them, an ordered region of undetermined secondary structure. When cysteine is scanned through the helices, characteristic repeating patterns of solvent exposure and burial are observed. Activity assays, both in vivo and in vitro, indicate that each helix possesses a buried packing face that is crucial for proper receptor function. The interhelical subdomain is at least partially buried and is also crucial for proper receptor function. Disulfide scanning places helix α4 distal to the central axis of the homodimer, while helix α5 is found to lie at the subunit interface. Finally, sequence alignments suggest that all three linker subdomains are highly conserved among the large subfamily of histidine kinase-coupled sensory receptors that possess methylation sites for use in covalent adaptation." @default.
• W2000290686 created "2016-06-24" @default.
• W2000290686 creator A5043739670 @default.
• W2000290686 creator A5052976771 @default.
• W2000290686 date "1998-07-01" @default.
• W2000290686 modified "2023-09-24" @default.
• W2000290686 title "Cysteine and Disulfide Scanning Reveals Two Amphiphilic Helices in the Linker Region of the Aspartate Chemoreceptor" @default.
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• W2000290686 cites W1509729044 @default.
• W2000290686 cites W1511132416 @default.
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• W2000290686 cites W1584903123 @default.
• W2000290686 cites W1595689634 @default.
• W2000290686 cites W1667106906 @default.
• W2000290686 cites W1838580001 @default.
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• W2000290686 cites W1963538333 @default.
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• W2000290686 cites W1972642574 @default.
• W2000290686 cites W1974361764 @default.
• W2000290686 cites W1976862382 @default.
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• W2000290686 cites W1979125268 @default.
• W2000290686 cites W1979347172 @default.
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• W2000290686 cites W1989732603 @default.
• W2000290686 cites W1993225645 @default.
• W2000290686 cites W1994522080 @default.
• W2000290686 cites W2000646546 @default.
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• W2000290686 cites W2047133733 @default.
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• W2000290686 cites W2049583838 @default.
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• W2000290686 cites W2059643036 @default.
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• W2000290686 cites W2177731497 @default.
• W2000290686 cites W2239384431 @default.
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• W2000290686 doi "https://doi.org/10.1021/bi980607g" @default.
• W2000290686 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2899697" @default.
• W2000290686 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9692965" @default.
• W2000290686 hasPublicationYear "1998" @default.
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