Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000297648> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2000297648 endingPage "272" @default.
- W2000297648 startingPage "269" @default.
- W2000297648 abstract "The activity of membrane-bound ecto-5'-nucleotidase and soluble e-Ns and c-N-II 5'-nucleotidases was evaluated on lymphocytes from patients affected by B-cell chronic lymphocytic leukemia (B-CLL). A statistically significative decrease in ecto-5'-nucleotidase, e-Ns, and c-N-II activities was observed in peripheral blood lymphocytes and in B and T populations from affected individuals.For the assay of ecto-5'-nucleotidase, e-Ns, and c-N-II activity we used a radioactive procedure coupled to HPLC. Since the ecto-5'-nucleotidase is identified as CD73 antigen, we performed immunofluorescence analysis using a specific monoclonal antibody. We analyzed ecto-5'-nucleotidase mRNA by RT-PCR to ascertain the possibility of an alteration in the transcription of its gene.A decrease in ecto-5'-nucleotidase activity was correlated to reduction in ecto-5'-nucleotidase positive cells (CD73+) in leukemia patients. RT-PCR produced a fragment of the expected size and the specific mRNA was found expressed in both healthy subjects and leukemia patients.The decrease in ecto-5'-nucleotidase activity in patients with B-CLL is not due to loss of transcription of the specific mRNA. The presence of point mutations, splicing alteration, or posttranslational modifications must be investigated. If a defect at DNA or RNA level will be detected, the molecular analysis will be considered for diagnosis of B-cell chronic lymphocytic leukemia." @default.
- W2000297648 created "2016-06-24" @default.
- W2000297648 creator A5004486169 @default.
- W2000297648 creator A5011079589 @default.
- W2000297648 creator A5016916859 @default.
- W2000297648 creator A5029071098 @default.
- W2000297648 creator A5068687462 @default.
- W2000297648 creator A5068983605 @default.
- W2000297648 creator A5078369536 @default.
- W2000297648 creator A5083539503 @default.
- W2000297648 creator A5086268455 @default.
- W2000297648 date "1998-06-01" @default.
- W2000297648 modified "2023-09-27" @default.
- W2000297648 title "5′-Nucleotidase Activity in Lymphocytes From Patients Affected by B-Cell Chronic Lymphocytic Leukemia" @default.
- W2000297648 cites W1546034604 @default.
- W2000297648 cites W1770545369 @default.
- W2000297648 cites W1792542720 @default.
- W2000297648 cites W1968237247 @default.
- W2000297648 cites W1980839596 @default.
- W2000297648 cites W2012683269 @default.
- W2000297648 cites W2028168883 @default.
- W2000297648 cites W2040818300 @default.
- W2000297648 cites W2048522953 @default.
- W2000297648 cites W2084214971 @default.
- W2000297648 cites W2108546120 @default.
- W2000297648 cites W2128226258 @default.
- W2000297648 cites W29459159 @default.
- W2000297648 cites W4293247451 @default.
- W2000297648 doi "https://doi.org/10.1016/s0009-9120(98)00017-4" @default.
- W2000297648 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9646951" @default.
- W2000297648 hasPublicationYear "1998" @default.
- W2000297648 type Work @default.
- W2000297648 sameAs 2000297648 @default.
- W2000297648 citedByCount "21" @default.
- W2000297648 countsByYear W20002976482013 @default.
- W2000297648 countsByYear W20002976482018 @default.
- W2000297648 crossrefType "journal-article" @default.
- W2000297648 hasAuthorship W2000297648A5004486169 @default.
- W2000297648 hasAuthorship W2000297648A5011079589 @default.
- W2000297648 hasAuthorship W2000297648A5016916859 @default.
- W2000297648 hasAuthorship W2000297648A5029071098 @default.
- W2000297648 hasAuthorship W2000297648A5068687462 @default.
- W2000297648 hasAuthorship W2000297648A5068983605 @default.
- W2000297648 hasAuthorship W2000297648A5078369536 @default.
- W2000297648 hasAuthorship W2000297648A5083539503 @default.
- W2000297648 hasAuthorship W2000297648A5086268455 @default.
- W2000297648 hasConcept C104317684 @default.
- W2000297648 hasConcept C153911025 @default.
- W2000297648 hasConcept C181199279 @default.
- W2000297648 hasConcept C185592680 @default.
- W2000297648 hasConcept C18880352 @default.
- W2000297648 hasConcept C203014093 @default.
- W2000297648 hasConcept C2777913276 @default.
- W2000297648 hasConcept C2777938653 @default.
- W2000297648 hasConcept C2778461978 @default.
- W2000297648 hasConcept C55493867 @default.
- W2000297648 hasConcept C86803240 @default.
- W2000297648 hasConceptScore W2000297648C104317684 @default.
- W2000297648 hasConceptScore W2000297648C153911025 @default.
- W2000297648 hasConceptScore W2000297648C181199279 @default.
- W2000297648 hasConceptScore W2000297648C185592680 @default.
- W2000297648 hasConceptScore W2000297648C18880352 @default.
- W2000297648 hasConceptScore W2000297648C203014093 @default.
- W2000297648 hasConceptScore W2000297648C2777913276 @default.
- W2000297648 hasConceptScore W2000297648C2777938653 @default.
- W2000297648 hasConceptScore W2000297648C2778461978 @default.
- W2000297648 hasConceptScore W2000297648C55493867 @default.
- W2000297648 hasConceptScore W2000297648C86803240 @default.
- W2000297648 hasIssue "4" @default.
- W2000297648 hasLocation W20002976481 @default.
- W2000297648 hasLocation W20002976482 @default.
- W2000297648 hasOpenAccess W2000297648 @default.
- W2000297648 hasPrimaryLocation W20002976481 @default.
- W2000297648 hasRelatedWork W1483680884 @default.
- W2000297648 hasRelatedWork W1978040782 @default.
- W2000297648 hasRelatedWork W1980455626 @default.
- W2000297648 hasRelatedWork W1991901616 @default.
- W2000297648 hasRelatedWork W2000297648 @default.
- W2000297648 hasRelatedWork W2411555213 @default.
- W2000297648 hasRelatedWork W2413979639 @default.
- W2000297648 hasRelatedWork W2437294827 @default.
- W2000297648 hasRelatedWork W2464949926 @default.
- W2000297648 hasRelatedWork W86156225 @default.
- W2000297648 hasVolume "31" @default.
- W2000297648 isParatext "false" @default.
- W2000297648 isRetracted "false" @default.
- W2000297648 magId "2000297648" @default.
- W2000297648 workType "article" @default.