Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000299154> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W2000299154 endingPage "S237" @default.
- W2000299154 startingPage "S232" @default.
- W2000299154 abstract "Endothelin (ET) receptor antagonists are nephroprotective in renal damage models of the rat. It is unknown whether ET receptor antagonists are also beneficial in human renal diseases. Major differences exist between the ET systems in rats and humans, therefore this study was designed to characterize the ET receptors expressed on human adult mesangial cells (HMCs). HMCs cultures are a surrogate model for the development of glomerulosclerosis. Binding experiments with [125I]ET-1 in the presence or the absence of the test compounds [endothelin-1, -3 (ET-1, ET-3), sarafotoxin 6c (S6c), or BQ123] revealed an affinity (IC50 values) of 10.5 nm for ET-1 and 87.6 nm for ET-3. The affinities of the ET(B) agonist S6c and the ET(A) antagonist BQ123 were 85.9 nm and > 10 microm, respectively. Thus, the ET receptor on HMCs shows an ET(B)-like pharmacology, but in contrast to the classical ET(B)-receptor the affinities are low. No affinity for BQ123 up to > 10 microm excludes the presence of ET(A)-receptors. Functional studies using microfluorimetry (fura-2 method) showed comparable biphasic calcium signals induced by 10 nm ET-1, ET-3 and S6c. This effect could not be inhibited by BQ123, but by the ET(B) antagonist BQ788. Reverse transcriptase polymerase chain reaction (RT-PCR) studies under different culture conditions showed that both ET(A)- and ET(B)-receptor mRNAs are expressed in HMCs. The amount of ET(A)-receptor mRNA increased 2.7-fold and that of the ET(B)-receptor mRNA 7.1-fold after stimulation with 10% fetal calf serum (FCS). ET-1, ET-3 and S6c stimulated HMCs growth (ET-1 > S6c > ET-3), but the magnitude of the effect of ET-1 is lower than reported in rat mesangial cells (rat MCs). The effect on HMCs growth could be inhibited by BQ788, but not by BQ123. Our data provide evidence for the expression of ET(B)-receptors on HMCs that are functionally active. This finding differs from the ET receptor expression in rat MCs as reported by others." @default.
- W2000299154 created "2016-06-24" @default.
- W2000299154 creator A5016185619 @default.
- W2000299154 creator A5022731076 @default.
- W2000299154 creator A5042152179 @default.
- W2000299154 creator A5051985691 @default.
- W2000299154 creator A5075578189 @default.
- W2000299154 creator A5081679951 @default.
- W2000299154 creator A5088446066 @default.
- W2000299154 date "2000-01-01" @default.
- W2000299154 modified "2023-10-16" @default.
- W2000299154 title "Adult Human Mesangial Cells (HMCs) Express Endothelin-B-Receptors which Mediate Endothelin-1-Induced Cell Growth" @default.
- W2000299154 doi "https://doi.org/10.1097/00005344-200036051-00069" @default.
- W2000299154 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11078385" @default.
- W2000299154 hasPublicationYear "2000" @default.
- W2000299154 type Work @default.
- W2000299154 sameAs 2000299154 @default.
- W2000299154 citedByCount "22" @default.
- W2000299154 countsByYear W20002991542012 @default.
- W2000299154 countsByYear W20002991542014 @default.
- W2000299154 countsByYear W20002991542015 @default.
- W2000299154 countsByYear W20002991542020 @default.
- W2000299154 countsByYear W20002991542023 @default.
- W2000299154 crossrefType "journal-article" @default.
- W2000299154 hasAuthorship W2000299154A5016185619 @default.
- W2000299154 hasAuthorship W2000299154A5022731076 @default.
- W2000299154 hasAuthorship W2000299154A5042152179 @default.
- W2000299154 hasAuthorship W2000299154A5051985691 @default.
- W2000299154 hasAuthorship W2000299154A5075578189 @default.
- W2000299154 hasAuthorship W2000299154A5081679951 @default.
- W2000299154 hasAuthorship W2000299154A5088446066 @default.
- W2000299154 hasConcept C126322002 @default.
- W2000299154 hasConcept C134018914 @default.
- W2000299154 hasConcept C144980905 @default.
- W2000299154 hasConcept C149601957 @default.
- W2000299154 hasConcept C153911025 @default.
- W2000299154 hasConcept C170493617 @default.
- W2000299154 hasConcept C185592680 @default.
- W2000299154 hasConcept C2776885963 @default.
- W2000299154 hasConcept C2778122271 @default.
- W2000299154 hasConcept C2778938600 @default.
- W2000299154 hasConcept C2779131090 @default.
- W2000299154 hasConcept C2780091579 @default.
- W2000299154 hasConcept C2909649922 @default.
- W2000299154 hasConcept C71924100 @default.
- W2000299154 hasConcept C86803240 @default.
- W2000299154 hasConceptScore W2000299154C126322002 @default.
- W2000299154 hasConceptScore W2000299154C134018914 @default.
- W2000299154 hasConceptScore W2000299154C144980905 @default.
- W2000299154 hasConceptScore W2000299154C149601957 @default.
- W2000299154 hasConceptScore W2000299154C153911025 @default.
- W2000299154 hasConceptScore W2000299154C170493617 @default.
- W2000299154 hasConceptScore W2000299154C185592680 @default.
- W2000299154 hasConceptScore W2000299154C2776885963 @default.
- W2000299154 hasConceptScore W2000299154C2778122271 @default.
- W2000299154 hasConceptScore W2000299154C2778938600 @default.
- W2000299154 hasConceptScore W2000299154C2779131090 @default.
- W2000299154 hasConceptScore W2000299154C2780091579 @default.
- W2000299154 hasConceptScore W2000299154C2909649922 @default.
- W2000299154 hasConceptScore W2000299154C71924100 @default.
- W2000299154 hasConceptScore W2000299154C86803240 @default.
- W2000299154 hasIssue "Supplement 1" @default.
- W2000299154 hasLocation W20002991541 @default.
- W2000299154 hasLocation W20002991542 @default.
- W2000299154 hasOpenAccess W2000299154 @default.
- W2000299154 hasPrimaryLocation W20002991541 @default.
- W2000299154 hasRelatedWork W1983371938 @default.
- W2000299154 hasRelatedWork W1995929592 @default.
- W2000299154 hasRelatedWork W2000299154 @default.
- W2000299154 hasRelatedWork W2001624788 @default.
- W2000299154 hasRelatedWork W2042710245 @default.
- W2000299154 hasRelatedWork W2092591926 @default.
- W2000299154 hasRelatedWork W2160355439 @default.
- W2000299154 hasRelatedWork W2253476305 @default.
- W2000299154 hasRelatedWork W4235046902 @default.
- W2000299154 hasRelatedWork W4247776882 @default.
- W2000299154 hasVolume "36" @default.
- W2000299154 isParatext "false" @default.
- W2000299154 isRetracted "false" @default.
- W2000299154 magId "2000299154" @default.
- W2000299154 workType "article" @default.