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- W2000299466 abstract "The secondary and tertiary structure of ribosomal 5 S RNAs has been studied using a variety of physical and chemical techniques (review [ 11). As part of our spectroscopic investigations on ribosomal RNAs we report here on Raman spectroscopic data of the native form of 5 S RNA of rat liver ribosomes. Laser Raman spectroscopy has proved to be a sensitive probe for the structure of RNAs, especially for the determination of the type and extent of base stacking, the amount of order in the ribophosphate backbone and the percentage of U residues in base-paired regions [2-51. Raman data have been published for E. coli 5 S RNA [5] and yeast 5 S RNA [6]. Our Raman data for rat liver 5 S RNA indicate a very high regularity of the ribophosphate backbone in the native form of rat liver 5 S RNA, comparable to native tRNAs. The stacking efficiency of the guanine ba.ses is much higher in 5 S RNA than in yeast tRNAPhe, while that of the adenine bases is lower. The results support a base pairing pattern [7] which is very similar to that proposed in [8]." @default.
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- W2000299466 date "1981-01-12" @default.
- W2000299466 modified "2023-10-18" @default.
- W2000299466 title "Laser Raman studies of rat liver ribosomal 5 S RNA" @default.
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- W2000299466 doi "https://doi.org/10.1016/0014-5793(81)80009-9" @default.
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