FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000300543> ?p ?o ?g. }

• W2000300543 endingPage "404" @default.
• W2000300543 startingPage "398" @default.
• W2000300543 abstract "We have subcloned the coding sequence for the Escherichia coli lysA gene coding for diaminopimelic acid decarboxylase (DAP decarboxylase) into a eukaryotic expression vector based on the SV40 early promoter. The activities of a series of constructs with different lengths of non-coding DNA at the 5′ and 3′ ends of the coding region have been compared by measuring the synthesis of lysine from diaminopimelic acid (DAP) in mouse 3T3 cells. A short non-coding sequence at the 3′ end reduced the expression of enzyme activity. Stable lines of 3T3 cells have been produced by co-transfection of the chimeric gene with a plasmid coding for G-418 resistance. Cells were grown in medium containing G-418 and resistant clones were screened for an ability to synthesise lysine from DAP. [3H]Lysine produced from [3H]DAP was incorporated into cell proteins. An enzyme extract from a cell line which had incorporated two copies of the gene synthesised 0.082 nmol of lysine / min per mg protein. In the intact cell the rate of lysine synthesis is limited by the uptake of DAP which is taken up at only 5% of the rate of lysine. lysA has a potential as a reporter gene in studies of gene expression in mammalian cells." @default.
• W2000300543 created "2016-06-24" @default.
• W2000300543 creator A5025540148 @default.
• W2000300543 creator A5060398628 @default.
• W2000300543 creator A5066952258 @default.
• W2000300543 date "1994-10-01" @default.
• W2000300543 modified "2023-09-25" @default.
• W2000300543 title "The expression of Escherichia coli diaminopimelate decarboxylase in mouse 3T3 cells" @default.
• W2000300543 cites W100287938 @default.
• W2000300543 cites W1007642911 @default.
• W2000300543 cites W120644162 @default.
• W2000300543 cites W1556094526 @default.
• W2000300543 cites W1574326311 @default.
• W2000300543 cites W1775749144 @default.
• W2000300543 cites W1967818736 @default.
• W2000300543 cites W1975574807 @default.
• W2000300543 cites W1993909070 @default.
• W2000300543 cites W2007511501 @default.
• W2000300543 cites W2168847939 @default.
• W2000300543 cites W2198958677 @default.
• W2000300543 cites W3181824 @default.
• W2000300543 cites W3214962068 @default.
• W2000300543 doi "https://doi.org/10.1016/0167-4781(94)90064-7" @default.
• W2000300543 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7918636" @default.
• W2000300543 hasPublicationYear "1994" @default.
• W2000300543 type Work @default.
• W2000300543 sameAs 2000300543 @default.
• W2000300543 citedByCount "6" @default.
• W2000300543 countsByYear W20003005432013 @default.
• W2000300543 countsByYear W20003005432014 @default.
• W2000300543 countsByYear W20003005432020 @default.
• W2000300543 crossrefType "journal-article" @default.
• W2000300543 hasAuthorship W2000300543A5025540148 @default.
• W2000300543 hasAuthorship W2000300543A5060398628 @default.
• W2000300543 hasAuthorship W2000300543A5066952258 @default.
• W2000300543 hasConcept C104317684 @default.
• W2000300543 hasConcept C150194340 @default.
• W2000300543 hasConcept C153911025 @default.
• W2000300543 hasConcept C161733203 @default.
• W2000300543 hasConcept C181199279 @default.
• W2000300543 hasConcept C22744801 @default.
• W2000300543 hasConcept C2776016237 @default.
• W2000300543 hasConcept C2776746612 @default.
• W2000300543 hasConcept C2776928777 @default.
• W2000300543 hasConcept C2777167505 @default.
• W2000300543 hasConcept C2779425345 @default.
• W2000300543 hasConcept C2779703059 @default.
• W2000300543 hasConcept C33161422 @default.
• W2000300543 hasConcept C40767141 @default.
• W2000300543 hasConcept C42062724 @default.
• W2000300543 hasConcept C515207424 @default.
• W2000300543 hasConcept C54009773 @default.
• W2000300543 hasConcept C54355233 @default.
• W2000300543 hasConcept C547475151 @default.
• W2000300543 hasConcept C55493867 @default.
• W2000300543 hasConcept C81885089 @default.
• W2000300543 hasConcept C86803240 @default.
• W2000300543 hasConcept C91779695 @default.
• W2000300543 hasConceptScore W2000300543C104317684 @default.
• W2000300543 hasConceptScore W2000300543C150194340 @default.
• W2000300543 hasConceptScore W2000300543C153911025 @default.
• W2000300543 hasConceptScore W2000300543C161733203 @default.
• W2000300543 hasConceptScore W2000300543C181199279 @default.
• W2000300543 hasConceptScore W2000300543C22744801 @default.
• W2000300543 hasConceptScore W2000300543C2776016237 @default.
• W2000300543 hasConceptScore W2000300543C2776746612 @default.
• W2000300543 hasConceptScore W2000300543C2776928777 @default.
• W2000300543 hasConceptScore W2000300543C2777167505 @default.
• W2000300543 hasConceptScore W2000300543C2779425345 @default.
• W2000300543 hasConceptScore W2000300543C2779703059 @default.
• W2000300543 hasConceptScore W2000300543C33161422 @default.
• W2000300543 hasConceptScore W2000300543C40767141 @default.
• W2000300543 hasConceptScore W2000300543C42062724 @default.
• W2000300543 hasConceptScore W2000300543C515207424 @default.
• W2000300543 hasConceptScore W2000300543C54009773 @default.
• W2000300543 hasConceptScore W2000300543C54355233 @default.
• W2000300543 hasConceptScore W2000300543C547475151 @default.
• W2000300543 hasConceptScore W2000300543C55493867 @default.
• W2000300543 hasConceptScore W2000300543C81885089 @default.
• W2000300543 hasConceptScore W2000300543C86803240 @default.
• W2000300543 hasConceptScore W2000300543C91779695 @default.
• W2000300543 hasIssue "2" @default.
• W2000300543 hasLocation W20003005431 @default.
• W2000300543 hasLocation W20003005432 @default.
• W2000300543 hasOpenAccess W2000300543 @default.
• W2000300543 hasPrimaryLocation W20003005431 @default.
• W2000300543 hasRelatedWork W1864650705 @default.
• W2000300543 hasRelatedWork W1989295391 @default.
• W2000300543 hasRelatedWork W2000300543 @default.
• W2000300543 hasRelatedWork W2050058362 @default.
• W2000300543 hasRelatedWork W2093818178 @default.
• W2000300543 hasRelatedWork W2360444651 @default.
• W2000300543 hasRelatedWork W2361843014 @default.
• W2000300543 hasRelatedWork W2380962287 @default.
• W2000300543 hasRelatedWork W2408959994 @default.
• W2000300543 hasRelatedWork W4254828962 @default.
• W2000300543 hasVolume "1219" @default.
• W2000300543 isParatext "false" @default.

• next