Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000301869> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2000301869 endingPage "2802" @default.
- W2000301869 startingPage "2796" @default.
- W2000301869 abstract "Voltage-gated sodium channels (VGSCs) are responsible for generating action potentials in nervous systems. Veratridine (VTD), a lipid soluble alkaloid isolated from sabadilla lily seed, is believed to bind to segment 6 of VGSCs and act as a partial agonist. However, high resolution structural interaction mechanism between VGSCs and VTD is difficult to elucidate because of the large size and membrane localization of VGSCs. Here, the authors designed model peptides corresponding to domain IV segment 6 (DIVS6) of rat skeletal muscle Na(v)1.4 and analyzed the complex of the model peptides and VTD by solution NMR analysis to obtain structural information of the interaction. The model peptides successfully formed an α-helices, which is the suspected native conformation of DIVS6, in aqueous 2,2,2-trifluoroethanol, a membrane-mimicking solvent. The VTD binding residues of the model peptide were identified using the NMR titration experiments with VTD, including a newly discovered VTD binding residue Leu14 (μ1-L1580 in Na(v)1.4), which has not been reported by point mutation studies. Mapping of VTD binding residues on the model peptide revealed the hydrophobic interaction surface. NMR titration experiments with a non-toxic analog of VTD, veracevine, also indicated that the steroidal backbone of VTD interacts with the hydrophobic interaction surface of DIVS6 and that the 3-acyl group of VTD possibly causes neurotoxicity by interacting with domain I segment 6 and/or domain IV segment 4." @default.
- W2000301869 created "2016-06-24" @default.
- W2000301869 creator A5004138216 @default.
- W2000301869 creator A5021926026 @default.
- W2000301869 creator A5038129551 @default.
- W2000301869 creator A5054073636 @default.
- W2000301869 date "2012-05-01" @default.
- W2000301869 modified "2023-10-16" @default.
- W2000301869 title "Solution NMR analysis of the binding mechanism of DIVS6 model peptides of voltage-gated sodium channels and the lipid soluble alkaloid veratridine" @default.
- W2000301869 cites W1583976079 @default.
- W2000301869 cites W1971857285 @default.
- W2000301869 cites W1983187084 @default.
- W2000301869 cites W2025586862 @default.
- W2000301869 cites W2029497985 @default.
- W2000301869 cites W2029667189 @default.
- W2000301869 cites W2041395543 @default.
- W2000301869 cites W2044521621 @default.
- W2000301869 cites W2061695857 @default.
- W2000301869 cites W2074666645 @default.
- W2000301869 cites W2075365473 @default.
- W2000301869 cites W2076550366 @default.
- W2000301869 cites W2077245165 @default.
- W2000301869 cites W2088899199 @default.
- W2000301869 cites W2089593580 @default.
- W2000301869 cites W2093985027 @default.
- W2000301869 cites W2098499866 @default.
- W2000301869 cites W2102575758 @default.
- W2000301869 cites W2104268112 @default.
- W2000301869 cites W2104415321 @default.
- W2000301869 cites W2127121086 @default.
- W2000301869 cites W2147979587 @default.
- W2000301869 cites W2152845515 @default.
- W2000301869 cites W2161137426 @default.
- W2000301869 cites W2168375170 @default.
- W2000301869 cites W2170671445 @default.
- W2000301869 cites W2317124745 @default.
- W2000301869 cites W2949367276 @default.
- W2000301869 cites W2043463975 @default.
- W2000301869 doi "https://doi.org/10.1016/j.bmc.2012.03.034" @default.
- W2000301869 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22483840" @default.
- W2000301869 hasPublicationYear "2012" @default.
- W2000301869 type Work @default.
- W2000301869 sameAs 2000301869 @default.
- W2000301869 citedByCount "6" @default.
- W2000301869 countsByYear W20003018692015 @default.
- W2000301869 countsByYear W20003018692017 @default.
- W2000301869 countsByYear W20003018692018 @default.
- W2000301869 crossrefType "journal-article" @default.
- W2000301869 hasAuthorship W2000301869A5004138216 @default.
- W2000301869 hasAuthorship W2000301869A5021926026 @default.
- W2000301869 hasAuthorship W2000301869A5038129551 @default.
- W2000301869 hasAuthorship W2000301869A5054073636 @default.
- W2000301869 hasConcept C107824862 @default.
- W2000301869 hasConcept C12554922 @default.
- W2000301869 hasConcept C178790620 @default.
- W2000301869 hasConcept C185592680 @default.
- W2000301869 hasConcept C2776986425 @default.
- W2000301869 hasConcept C2779281246 @default.
- W2000301869 hasConcept C50952357 @default.
- W2000301869 hasConcept C537181965 @default.
- W2000301869 hasConcept C55493867 @default.
- W2000301869 hasConcept C71240020 @default.
- W2000301869 hasConcept C86803240 @default.
- W2000301869 hasConceptScore W2000301869C107824862 @default.
- W2000301869 hasConceptScore W2000301869C12554922 @default.
- W2000301869 hasConceptScore W2000301869C178790620 @default.
- W2000301869 hasConceptScore W2000301869C185592680 @default.
- W2000301869 hasConceptScore W2000301869C2776986425 @default.
- W2000301869 hasConceptScore W2000301869C2779281246 @default.
- W2000301869 hasConceptScore W2000301869C50952357 @default.
- W2000301869 hasConceptScore W2000301869C537181965 @default.
- W2000301869 hasConceptScore W2000301869C55493867 @default.
- W2000301869 hasConceptScore W2000301869C71240020 @default.
- W2000301869 hasConceptScore W2000301869C86803240 @default.
- W2000301869 hasIssue "9" @default.
- W2000301869 hasLocation W20003018691 @default.
- W2000301869 hasLocation W20003018692 @default.
- W2000301869 hasOpenAccess W2000301869 @default.
- W2000301869 hasPrimaryLocation W20003018691 @default.
- W2000301869 hasRelatedWork W1979777090 @default.
- W2000301869 hasRelatedWork W2001751492 @default.
- W2000301869 hasRelatedWork W2018155809 @default.
- W2000301869 hasRelatedWork W2024248286 @default.
- W2000301869 hasRelatedWork W2034692915 @default.
- W2000301869 hasRelatedWork W2054706865 @default.
- W2000301869 hasRelatedWork W2088721615 @default.
- W2000301869 hasRelatedWork W2340291223 @default.
- W2000301869 hasRelatedWork W4205205905 @default.
- W2000301869 hasRelatedWork W4220789546 @default.
- W2000301869 hasVolume "20" @default.
- W2000301869 isParatext "false" @default.
- W2000301869 isRetracted "false" @default.
- W2000301869 magId "2000301869" @default.
- W2000301869 workType "article" @default.