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- W2000302248 abstract "We found small GTP-binding proteins in the outer envelope membrane of pea chloroplasts. The proteins in this membrane were separated by SDS-PAGE, transferred to a nitrocellulose filter, and incubated with [α-23P]GTP. Three GTP-binding proteins with the molecular weight of 24 000 were found. Binding was prevented by 10−8—10−7 M GTP or by 10−7 M guanosine 5′-[γ-thio]triphosphate or GDP; binding was unaffected by 10−8—10−6 M ATP. Thermolysin treatment of intact chloroplasts resulted in the loss of GTP-binding activity, suggesting that these proteins were in the cytosolic side of the outer envelope membrane." @default.
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- W2000302248 date "1991-11-01" @default.
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- W2000302248 title "Detection of small GTP-binding proteins in the outer envelope membrane of pea chloroplasts" @default.
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- W2000302248 doi "https://doi.org/10.1016/0014-5793(91)81166-6" @default.
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