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- W2000307731 abstract "Abstract A new assay procedure has been developed for methotrexate in plasma samples, using high-pressure liquid chromatography with fluorescence detection. A novel feature of this procedure is the reduction of methotrexate with dithionite to yield a highly fluorescent product, tentatively identified as 2,4-diamino-6-methylpteridine. Simultaneous detection of citrovorum factor (folinic acid) by fluorescence has also been demonstrated, and detection of vincristine by uv absorption. The procedure is suitable for simultaneous measurement of methotrexate, citrovorum factor, and vincristine in plasma samples at concentrations as low as 5 × 10−9, 10−6, and 10−7 m , respectively. Sample preparation time is approximately 45 min, and elution time about 30 min." @default.
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- W2000307731 date "1981-07-01" @default.
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- W2000307731 title "Assay for methotrexate in nanomolar concentrations with simultaneous detection of citrovorum factor and vincristine" @default.
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- W2000307731 doi "https://doi.org/10.1016/0003-2697(81)90493-0" @default.
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