Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000314280> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2000314280 endingPage "16" @default.
- W2000314280 startingPage "13" @default.
- W2000314280 abstract "A method for an ultrasensitive silver-based color staining of proteins described by Sammons et al. was applied to the detection of oligonucleotides on polyacrylamide gels with slight modifications in the method of fixation. With these modifications, we can stain even tetranucleotide bands such as dA4. Furthermore, the sensitivity of staining of Hae III fragments of φX 174 RF DNA is several times higher than with ethydium bromide staining. The sensitivity of this silver-staining differs markedly among various homo-oligonucleotides. The limit of detection of rA10 or dA10 is approximately 40pg per mm2 of cross-sectional area. Homo-oligonucleotides, dG10 and dC10, are also detectable. However, the dT10 band concentrated more than 1, 000-fold could not be detected on the polyacrylamide gel by silver staining. Thus, the silver staining method is very useful for the sensitive detection of oligonucleotides as short as tetranucleotides that are not stained by ethidium bromide." @default.
- W2000314280 created "2016-06-24" @default.
- W2000314280 creator A5011818455 @default.
- W2000314280 creator A5014344295 @default.
- W2000314280 creator A5028559781 @default.
- W2000314280 creator A5055387930 @default.
- W2000314280 date "1999-01-01" @default.
- W2000314280 modified "2023-10-16" @default.
- W2000314280 title "Ultrasensitive silver staining of oligonucleotides." @default.
- W2000314280 cites W1984592936 @default.
- W2000314280 cites W1988345272 @default.
- W2000314280 cites W1996071792 @default.
- W2000314280 cites W2001980216 @default.
- W2000314280 cites W2003216620 @default.
- W2000314280 cites W2022961230 @default.
- W2000314280 cites W2044936649 @default.
- W2000314280 cites W2071235278 @default.
- W2000314280 cites W2094082118 @default.
- W2000314280 cites W2101948512 @default.
- W2000314280 cites W2152193561 @default.
- W2000314280 doi "https://doi.org/10.2198/sbk.43.13" @default.
- W2000314280 hasPublicationYear "1999" @default.
- W2000314280 type Work @default.
- W2000314280 sameAs 2000314280 @default.
- W2000314280 citedByCount "0" @default.
- W2000314280 crossrefType "journal-article" @default.
- W2000314280 hasAuthorship W2000314280A5011818455 @default.
- W2000314280 hasAuthorship W2000314280A5014344295 @default.
- W2000314280 hasAuthorship W2000314280A5028559781 @default.
- W2000314280 hasAuthorship W2000314280A5055387930 @default.
- W2000314280 hasBestOaLocation W20003142801 @default.
- W2000314280 hasConcept C101660505 @default.
- W2000314280 hasConcept C119128265 @default.
- W2000314280 hasConcept C129312508 @default.
- W2000314280 hasConcept C153911025 @default.
- W2000314280 hasConcept C181199279 @default.
- W2000314280 hasConcept C185592680 @default.
- W2000314280 hasConcept C25330733 @default.
- W2000314280 hasConcept C2776907736 @default.
- W2000314280 hasConcept C2778862431 @default.
- W2000314280 hasConcept C2781294515 @default.
- W2000314280 hasConcept C43617362 @default.
- W2000314280 hasConcept C54355233 @default.
- W2000314280 hasConcept C552990157 @default.
- W2000314280 hasConcept C55493867 @default.
- W2000314280 hasConcept C74864618 @default.
- W2000314280 hasConcept C86803240 @default.
- W2000314280 hasConceptScore W2000314280C101660505 @default.
- W2000314280 hasConceptScore W2000314280C119128265 @default.
- W2000314280 hasConceptScore W2000314280C129312508 @default.
- W2000314280 hasConceptScore W2000314280C153911025 @default.
- W2000314280 hasConceptScore W2000314280C181199279 @default.
- W2000314280 hasConceptScore W2000314280C185592680 @default.
- W2000314280 hasConceptScore W2000314280C25330733 @default.
- W2000314280 hasConceptScore W2000314280C2776907736 @default.
- W2000314280 hasConceptScore W2000314280C2778862431 @default.
- W2000314280 hasConceptScore W2000314280C2781294515 @default.
- W2000314280 hasConceptScore W2000314280C43617362 @default.
- W2000314280 hasConceptScore W2000314280C54355233 @default.
- W2000314280 hasConceptScore W2000314280C552990157 @default.
- W2000314280 hasConceptScore W2000314280C55493867 @default.
- W2000314280 hasConceptScore W2000314280C74864618 @default.
- W2000314280 hasConceptScore W2000314280C86803240 @default.
- W2000314280 hasIssue "1" @default.
- W2000314280 hasLocation W20003142801 @default.
- W2000314280 hasOpenAccess W2000314280 @default.
- W2000314280 hasPrimaryLocation W20003142801 @default.
- W2000314280 hasRelatedWork W1988345272 @default.
- W2000314280 hasRelatedWork W2000314280 @default.
- W2000314280 hasRelatedWork W2011633440 @default.
- W2000314280 hasRelatedWork W2026729683 @default.
- W2000314280 hasRelatedWork W2115914234 @default.
- W2000314280 hasRelatedWork W2400795186 @default.
- W2000314280 hasRelatedWork W2414951827 @default.
- W2000314280 hasRelatedWork W2472684825 @default.
- W2000314280 hasRelatedWork W2939536591 @default.
- W2000314280 hasRelatedWork W4290146197 @default.
- W2000314280 hasVolume "43" @default.
- W2000314280 isParatext "false" @default.
- W2000314280 isRetracted "false" @default.
- W2000314280 magId "2000314280" @default.
- W2000314280 workType "article" @default.