FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000314728> ?p ?o ?g. }

• W2000314728 endingPage "e43877" @default.
• W2000314728 startingPage "e43877" @default.
• W2000314728 abstract "The mechanism of inactivation of human enzyme N-acylethanolamine-hydrolyzing acid amidase (hNAAA), with selected inhibitors identified in a novel fluorescent based assay developed for characterization of both reversible and irreversible inhibitors, was investigated kinetically and using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). 1-Isothiocyanatopentadecane (AM9023) was found to be a potent, selective and reversible hNAAA inhibitor, while two others, 5-((biphenyl-4-yl)methyl)-N,N-dimethyl-2H-tetrazole-2-carboxamide (AM6701) and N-Benzyloxycarbonyl-L-serine β-lactone (N-Cbz-serine β-lactone), inhibited hNAAA in a covalent and irreversible manner. MS analysis of the hNAAA/covalent inhibitor complexes identified modification only of the N-terminal cysteine (Cys126) of the β-subunit, confirming a suggested mechanism of hNAAA inactivation by the β-lactone containing inhibitors. These experiments provide direct evidence of the key role of Cys126 in hNAAA inactivation by different classes of covalent inhibitors, confirming the essential role of cysteine for catalysis and inhibition in this cysteine N-terminal nucleophile hydrolase enzyme. They also provide a methodology for the rapid screening and characterization of large libraries of compounds as potential inhibitors of NAAA, and subsequent characterization or their mechanism through MALDI-TOF MS based bottom up-proteomics." @default.
• W2000314728 created "2016-06-24" @default.
• W2000314728 creator A5000540926 @default.
• W2000314728 creator A5001517015 @default.
• W2000314728 creator A5036728759 @default.
• W2000314728 creator A5054161072 @default.
• W2000314728 creator A5072715661 @default.
• W2000314728 creator A5081335529 @default.
• W2000314728 date "2012-08-31" @default.
• W2000314728 modified "2023-09-26" @default.
• W2000314728 title "Biochemical and Mass Spectrometric Characterization of Human N-Acylethanolamine-Hydrolyzing Acid Amidase Inhibition" @default.
• W2000314728 cites W1550877969 @default.
• W2000314728 cites W1972891843 @default.
• W2000314728 cites W1974372915 @default.
• W2000314728 cites W1985164787 @default.
• W2000314728 cites W1986015202 @default.
• W2000314728 cites W1986655449 @default.
• W2000314728 cites W1989432011 @default.
• W2000314728 cites W1995793646 @default.
• W2000314728 cites W1996767019 @default.
• W2000314728 cites W2002355116 @default.
• W2000314728 cites W2008028424 @default.
• W2000314728 cites W2012648927 @default.
• W2000314728 cites W2018391753 @default.
• W2000314728 cites W2020091120 @default.
• W2000314728 cites W2023259667 @default.
• W2000314728 cites W2026874594 @default.
• W2000314728 cites W2042676931 @default.
• W2000314728 cites W2043559802 @default.
• W2000314728 cites W2045721135 @default.
• W2000314728 cites W2045872436 @default.
• W2000314728 cites W2053648339 @default.
• W2000314728 cites W2064601706 @default.
• W2000314728 cites W2082286813 @default.
• W2000314728 cites W2083616159 @default.
• W2000314728 cites W2085673938 @default.
• W2000314728 cites W2085870291 @default.
• W2000314728 cites W2088749908 @default.
• W2000314728 cites W2090660376 @default.
• W2000314728 cites W2095720174 @default.
• W2000314728 cites W2100692971 @default.
• W2000314728 cites W2108198026 @default.
• W2000314728 cites W2120367130 @default.
• W2000314728 cites W2138070531 @default.
• W2000314728 cites W2153187042 @default.
• W2000314728 cites W2165128851 @default.
• W2000314728 cites W2170775670 @default.
• W2000314728 cites W3151315634 @default.
• W2000314728 doi "https://doi.org/10.1371/journal.pone.0043877" @default.
• W2000314728 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3432061" @default.
• W2000314728 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22952796" @default.
• W2000314728 hasPublicationYear "2012" @default.
• W2000314728 type Work @default.
• W2000314728 sameAs 2000314728 @default.
• W2000314728 citedByCount "34" @default.
• W2000314728 countsByYear W20003147282013 @default.
• W2000314728 countsByYear W20003147282014 @default.
• W2000314728 countsByYear W20003147282015 @default.
• W2000314728 countsByYear W20003147282016 @default.
• W2000314728 countsByYear W20003147282017 @default.
• W2000314728 countsByYear W20003147282018 @default.
• W2000314728 countsByYear W20003147282020 @default.
• W2000314728 countsByYear W20003147282021 @default.
• W2000314728 countsByYear W20003147282023 @default.
• W2000314728 crossrefType "journal-article" @default.
• W2000314728 hasAuthorship W2000314728A5000540926 @default.
• W2000314728 hasAuthorship W2000314728A5001517015 @default.
• W2000314728 hasAuthorship W2000314728A5036728759 @default.
• W2000314728 hasAuthorship W2000314728A5054161072 @default.
• W2000314728 hasAuthorship W2000314728A5072715661 @default.
• W2000314728 hasAuthorship W2000314728A5081335529 @default.
• W2000314728 hasBestOaLocation W20003147281 @default.
• W2000314728 hasConcept C181199279 @default.
• W2000314728 hasConcept C185592680 @default.
• W2000314728 hasConcept C2776414213 @default.
• W2000314728 hasConcept C2779201268 @default.
• W2000314728 hasConcept C55493867 @default.
• W2000314728 hasConcept C65588867 @default.
• W2000314728 hasConcept C71240020 @default.
• W2000314728 hasConceptScore W2000314728C181199279 @default.
• W2000314728 hasConceptScore W2000314728C185592680 @default.
• W2000314728 hasConceptScore W2000314728C2776414213 @default.
• W2000314728 hasConceptScore W2000314728C2779201268 @default.
• W2000314728 hasConceptScore W2000314728C55493867 @default.
• W2000314728 hasConceptScore W2000314728C65588867 @default.
• W2000314728 hasConceptScore W2000314728C71240020 @default.
• W2000314728 hasIssue "8" @default.
• W2000314728 hasLocation W20003147281 @default.
• W2000314728 hasLocation W20003147282 @default.
• W2000314728 hasLocation W20003147283 @default.
• W2000314728 hasLocation W20003147284 @default.
• W2000314728 hasOpenAccess W2000314728 @default.
• W2000314728 hasPrimaryLocation W20003147281 @default.
• W2000314728 hasRelatedWork W1173353821 @default.
• W2000314728 hasRelatedWork W1574163474 @default.
• W2000314728 hasRelatedWork W1969765414 @default.
• W2000314728 hasRelatedWork W2042971445 @default.
• W2000314728 hasRelatedWork W2046311107 @default.

• next