Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000316898> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2000316898 endingPage "275" @default.
- W2000316898 startingPage "270" @default.
- W2000316898 abstract "To isolate chitinases and β-1,3-glucanases from the intercellular space of oats (Avena sativa L.), primary leaves were infiltrated with buffer and subjected to gentle centrifugation to obtain intercellular washing fluid (IWF). Approximately 5% of the chitinase and 10% of the β-1,3-glucanase activity of the whole leaf were released. Only small amounts (0.01-0.03%) of the intracellular marker malate-dehydrogenase were released into the IWF during infiltration. Activities of chitinase and β-1,3-glucanase in the IWF and in the leaf extract were compared by different chromatographic methods. On Sephadex G-75, chitinase appeared as a single peak (M r 29.8 kD) both in IWF and homogenate. β-1,3-Glucanase, however, showed two peaks in the IWF (M r 52 and 31.3 kD), whereas the elution pattern of the homogenate showed only one major peak at 22 kD. Chromatofocusing indicated that the IWF contained four chitinases and five β-1,3-glucanases. The elution pattern of the homogenate and IWF were similar with regard to the elution pH, but the peak intensities were distinctly different. Our results demonstrate that extracellular β-1,3-glucanases are different from those located intracellularly. Extracellular and intracellular chitinases do not differ in molecular properties, except for one isozyme which seems to be confined to the extracellular space. We suggest that both enzymes might play a special role in pathogenesis during fungal infection." @default.
- W2000316898 created "2016-06-24" @default.
- W2000316898 creator A5050168442 @default.
- W2000316898 creator A5058421495 @default.
- W2000316898 creator A5068826213 @default.
- W2000316898 date "1988-10-01" @default.
- W2000316898 modified "2023-10-12" @default.
- W2000316898 title "Chitinases and β-1,3-Glucanases in the Apoplastic Compartment of Oat Leaves (<i>Avena sativa</i> L.)" @default.
- W2000316898 cites W1535002104 @default.
- W2000316898 cites W1568028271 @default.
- W2000316898 cites W1653139269 @default.
- W2000316898 cites W1988117592 @default.
- W2000316898 cites W2038858425 @default.
- W2000316898 cites W2060935726 @default.
- W2000316898 cites W2070933594 @default.
- W2000316898 cites W2080494810 @default.
- W2000316898 cites W2087229363 @default.
- W2000316898 cites W2100837269 @default.
- W2000316898 cites W2119460480 @default.
- W2000316898 cites W2128635872 @default.
- W2000316898 doi "https://doi.org/10.1104/pp.88.2.270" @default.
- W2000316898 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1055567" @default.
- W2000316898 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16666294" @default.
- W2000316898 hasPublicationYear "1988" @default.
- W2000316898 type Work @default.
- W2000316898 sameAs 2000316898 @default.
- W2000316898 citedByCount "109" @default.
- W2000316898 countsByYear W20003168982012 @default.
- W2000316898 countsByYear W20003168982013 @default.
- W2000316898 countsByYear W20003168982014 @default.
- W2000316898 countsByYear W20003168982015 @default.
- W2000316898 countsByYear W20003168982018 @default.
- W2000316898 countsByYear W20003168982019 @default.
- W2000316898 countsByYear W20003168982020 @default.
- W2000316898 countsByYear W20003168982021 @default.
- W2000316898 countsByYear W20003168982022 @default.
- W2000316898 countsByYear W20003168982023 @default.
- W2000316898 crossrefType "journal-article" @default.
- W2000316898 hasAuthorship W2000316898A5050168442 @default.
- W2000316898 hasAuthorship W2000316898A5058421495 @default.
- W2000316898 hasAuthorship W2000316898A5068826213 @default.
- W2000316898 hasBestOaLocation W20003168982 @default.
- W2000316898 hasConcept C163994747 @default.
- W2000316898 hasConcept C173749725 @default.
- W2000316898 hasConcept C181199279 @default.
- W2000316898 hasConcept C185592680 @default.
- W2000316898 hasConcept C193363816 @default.
- W2000316898 hasConcept C2776829962 @default.
- W2000316898 hasConcept C2779530776 @default.
- W2000316898 hasConcept C2779908699 @default.
- W2000316898 hasConcept C2780778831 @default.
- W2000316898 hasConcept C28406088 @default.
- W2000316898 hasConcept C43617362 @default.
- W2000316898 hasConcept C55493867 @default.
- W2000316898 hasConcept C59822182 @default.
- W2000316898 hasConcept C79879829 @default.
- W2000316898 hasConcept C86803240 @default.
- W2000316898 hasConceptScore W2000316898C163994747 @default.
- W2000316898 hasConceptScore W2000316898C173749725 @default.
- W2000316898 hasConceptScore W2000316898C181199279 @default.
- W2000316898 hasConceptScore W2000316898C185592680 @default.
- W2000316898 hasConceptScore W2000316898C193363816 @default.
- W2000316898 hasConceptScore W2000316898C2776829962 @default.
- W2000316898 hasConceptScore W2000316898C2779530776 @default.
- W2000316898 hasConceptScore W2000316898C2779908699 @default.
- W2000316898 hasConceptScore W2000316898C2780778831 @default.
- W2000316898 hasConceptScore W2000316898C28406088 @default.
- W2000316898 hasConceptScore W2000316898C43617362 @default.
- W2000316898 hasConceptScore W2000316898C55493867 @default.
- W2000316898 hasConceptScore W2000316898C59822182 @default.
- W2000316898 hasConceptScore W2000316898C79879829 @default.
- W2000316898 hasConceptScore W2000316898C86803240 @default.
- W2000316898 hasIssue "2" @default.
- W2000316898 hasLocation W20003168981 @default.
- W2000316898 hasLocation W20003168982 @default.
- W2000316898 hasLocation W20003168983 @default.
- W2000316898 hasLocation W20003168984 @default.
- W2000316898 hasOpenAccess W2000316898 @default.
- W2000316898 hasPrimaryLocation W20003168981 @default.
- W2000316898 hasRelatedWork W1517443334 @default.
- W2000316898 hasRelatedWork W2000316898 @default.
- W2000316898 hasRelatedWork W2024310672 @default.
- W2000316898 hasRelatedWork W2074186887 @default.
- W2000316898 hasRelatedWork W2091795172 @default.
- W2000316898 hasRelatedWork W2092373033 @default.
- W2000316898 hasRelatedWork W2134012443 @default.
- W2000316898 hasRelatedWork W2145008199 @default.
- W2000316898 hasRelatedWork W2414874605 @default.
- W2000316898 hasRelatedWork W49443560 @default.
- W2000316898 hasVolume "88" @default.
- W2000316898 isParatext "false" @default.
- W2000316898 isRetracted "false" @default.
- W2000316898 magId "2000316898" @default.
- W2000316898 workType "article" @default.