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- W2000320683 abstract "We describe a new method for calibrating optical trapping measurements in which tension is applied in the direction of the laser beam to a molecule tethered between a surface and an optically trapped bead. Specifically, we present a generally-applicable procedure for converting from the measured scattering intensity and the measured stage displacement to applied tension and bead-coverslip separation, using measurements of the light intensity scattered from an untethered, trapped bead. Our calibration accounts for a number of effects, including aberrations and the interference of forward-reflected bead-scattered light with the trapping beam. To demonstrate the accuracy of our method, we show measurements of the DNA force-versus-extension relation using a range of laser intensities, and show that these measurements match the expected extensible wormlike-chain (WLC) behavior. Finally, we also demonstrate a force-clamp, in which the tension in a tether is held fixed while the extension varies as a result of molecular events." @default.
- W2000320683 created "2016-06-24" @default.
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- W2000320683 date "2012-10-01" @default.
- W2000320683 modified "2023-10-16" @default.
- W2000320683 title "Practical axial optical trapping" @default.
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- W2000320683 doi "https://doi.org/10.1063/1.4757862" @default.
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