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- W2000323861 abstract "Ussing chambers are frequently used for in vitro evaluation of intestinal transport physiology. The current study describes investigating the jejunal tissue from laying hens using a specific preparation method and evaluates the effect of glutamine in the maintenance buffer. Tunica mucosa was stripped from 104 jejunal samples from 10 hens and stabilised by a net device. Fifty samples were maintained with modified Krebs–Henseleit buffer (Control), 54 samples with additional 5 mM glutamine (Group Gln). The percentage of responding samples varied between 87 and 100%. Mean short circuit current (ΔI sc,) [µA/cm2] of samples exposed to 10 mM glucose in the Control group and Group Gln was 17.0 and 14.6 (p = 0.836), respectively, of samples exposed to 100 µM phloridzin –13.3 and –11.8 (p = 0.712), respectively, and of samples exposed to 100 µM carbachol 4.7 and 3.7 (p = 0.450), respectively. In conclusion, the net-supported method enabled a reliable investigation of jejunum from laying hens. Glutamine in the maintenance buffer was of no significant benefit." @default.
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- W2000323861 date "2013-04-01" @default.
- W2000323861 modified "2023-10-03" @default.
- W2000323861 title "Method for the preparation of mucosal flaps from the jejunum of laying hens for transporter studies in Ussing chambers" @default.
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- W2000323861 doi "https://doi.org/10.1080/1745039x.2013.776328" @default.
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