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• W2000323876 abstract "Miniaturization in biological analyses has several advantages, such as sample volume reduction and fast response time. The integration of miniaturized biosensors within lab-on-a-chip setups under flow conditions is highly desirable, not only because it simplifies process handling but also because measurements become more robust and operator-independent. In this work, we study the integration of flow amperometric biosensors within a microfluidic platform when analyte concentration is indirectly measured. As a case study, we used a platinum miniaturized glucose biosensor, where glucose is enzymatically converted to H2O2 that is oxidized at the electrode. The experimental results produced are strongly coupled to a theoretical analysis of fluid dynamic conditions affecting the electrochemical response of the sensor. We verified that the choice of the inlet flow rate is a critical parameter in flow biosensors, because it affects both glucose and H2O2 transport, to and from the electrode. We identify optimal flow rate conditions for accurate sensing at high time resolution. A dimensionless theoretical analysis allows the extension of the results to other sensing systems according to fluid dynamic similarity principles. Furthermore, we developed a microfluidic design that connects a sampling unit to the biosensor, in order to decouple the sampling flow rate from that of the actual measurement." @default.
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• W2000323876 date "2012-04-20" @default.
• W2000323876 modified "2023-10-14" @default.
• W2000323876 title "Flow biosensing and sampling in indirect electrochemical detection" @default.
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• W2000323876 doi "https://doi.org/10.1063/1.4705368" @default.
• W2000323876 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3360726" @default.
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