Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000331444> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2000331444 endingPage "259" @default.
- W2000331444 startingPage "253" @default.
- W2000331444 abstract "In a previous work, we have described the tryptic cleavage of yeast flavocytochrome b2 into its two functional domains: a cytochrome b2 core and a flavodehydrogenase. The lactate dehydrogenase efficiency of the latter was, however, dramatically low, only about 1% that of intact flavocytochrome b2. Our present study concerns a new flavodehydrogenase derivative of Hansenula anomala flavocytochrome b2 which spontaneously dissociates from the cytochrome domain when the polypeptide bridge connecting them is cleaved by Staphylococcus aureus V8 protease I. This flavodehydrogenase was purified and some of its functional and structural properties were studied. It presents an exceptionally high lactate dehydrogenase activity, about 80% that of flavocytochrome b2. This result clearly demonstrates that the cytochrome domain is not necessary for the lactate dehydrogenase function and suggests an autonomous folding for both domains. Our results are discussed in terms of ‘gene fusion’." @default.
- W2000331444 created "2016-06-24" @default.
- W2000331444 creator A5006799133 @default.
- W2000331444 creator A5008622742 @default.
- W2000331444 creator A5034981357 @default.
- W2000331444 date "1983-02-01" @default.
- W2000331444 modified "2023-10-03" @default.
- W2000331444 title "Proteolytic Cleavage of Hansenula anomala Flavocytochrome b2 into Its Two Functional Domains. Isolation of a Highly Active Flavodehydrogenase and a Cytochrome b2 Core" @default.
- W2000331444 cites W1000655315 @default.
- W2000331444 cites W1499864109 @default.
- W2000331444 cites W1538410954 @default.
- W2000331444 cites W1545499967 @default.
- W2000331444 cites W1549634513 @default.
- W2000331444 cites W1594796409 @default.
- W2000331444 cites W1598365414 @default.
- W2000331444 cites W1974774743 @default.
- W2000331444 cites W1977555686 @default.
- W2000331444 cites W1991449903 @default.
- W2000331444 cites W2003564611 @default.
- W2000331444 cites W2009114559 @default.
- W2000331444 cites W2027147718 @default.
- W2000331444 cites W2050761271 @default.
- W2000331444 cites W2052714082 @default.
- W2000331444 cites W2061277938 @default.
- W2000331444 cites W2065036876 @default.
- W2000331444 cites W2068861224 @default.
- W2000331444 cites W2069311205 @default.
- W2000331444 cites W2082522919 @default.
- W2000331444 cites W2084986748 @default.
- W2000331444 cites W2089772069 @default.
- W2000331444 cites W2119948031 @default.
- W2000331444 cites W942049167 @default.
- W2000331444 doi "https://doi.org/10.1111/j.1432-1033.1983.tb07144.x" @default.
- W2000331444 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6337844" @default.
- W2000331444 hasPublicationYear "1983" @default.
- W2000331444 type Work @default.
- W2000331444 sameAs 2000331444 @default.
- W2000331444 citedByCount "33" @default.
- W2000331444 crossrefType "journal-article" @default.
- W2000331444 hasAuthorship W2000331444A5006799133 @default.
- W2000331444 hasAuthorship W2000331444A5008622742 @default.
- W2000331444 hasAuthorship W2000331444A5034981357 @default.
- W2000331444 hasBestOaLocation W20003314441 @default.
- W2000331444 hasConcept C181199279 @default.
- W2000331444 hasConcept C185592680 @default.
- W2000331444 hasConcept C2776714187 @default.
- W2000331444 hasConcept C2779222958 @default.
- W2000331444 hasConcept C2780768313 @default.
- W2000331444 hasConcept C28859421 @default.
- W2000331444 hasConcept C29311851 @default.
- W2000331444 hasConcept C55493867 @default.
- W2000331444 hasConcept C71240020 @default.
- W2000331444 hasConcept C86803240 @default.
- W2000331444 hasConceptScore W2000331444C181199279 @default.
- W2000331444 hasConceptScore W2000331444C185592680 @default.
- W2000331444 hasConceptScore W2000331444C2776714187 @default.
- W2000331444 hasConceptScore W2000331444C2779222958 @default.
- W2000331444 hasConceptScore W2000331444C2780768313 @default.
- W2000331444 hasConceptScore W2000331444C28859421 @default.
- W2000331444 hasConceptScore W2000331444C29311851 @default.
- W2000331444 hasConceptScore W2000331444C55493867 @default.
- W2000331444 hasConceptScore W2000331444C71240020 @default.
- W2000331444 hasConceptScore W2000331444C86803240 @default.
- W2000331444 hasIssue "2" @default.
- W2000331444 hasLocation W20003314441 @default.
- W2000331444 hasLocation W20003314442 @default.
- W2000331444 hasOpenAccess W2000331444 @default.
- W2000331444 hasPrimaryLocation W20003314441 @default.
- W2000331444 hasRelatedWork W1859783629 @default.
- W2000331444 hasRelatedWork W2000554875 @default.
- W2000331444 hasRelatedWork W2009424100 @default.
- W2000331444 hasRelatedWork W2054061426 @default.
- W2000331444 hasRelatedWork W2060237942 @default.
- W2000331444 hasRelatedWork W2125012650 @default.
- W2000331444 hasRelatedWork W2343960475 @default.
- W2000331444 hasRelatedWork W2403043090 @default.
- W2000331444 hasRelatedWork W2491451478 @default.
- W2000331444 hasRelatedWork W4229581964 @default.
- W2000331444 hasVolume "130" @default.
- W2000331444 isParatext "false" @default.
- W2000331444 isRetracted "false" @default.
- W2000331444 magId "2000331444" @default.
- W2000331444 workType "article" @default.