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• W2000335112 abstract "The novel TiO 2 /graphene sheet (TiO 2 /GSs) nanocomposites were synthesized using graphene oxide sheets and TiCl 3 . All target composites were characterized by transmission electron microscopy (TEM), X-ray electron diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and energy dispersive X-ray spectra (EDX), and the surface areas were tested with the Brunauer-Emmett-Teller (BET) method. We studied the cytotoxicity of TiO 2 /GSs nanoparticles on A549 cells by examining the influence of TiO 2 /GSs on cell morphology and viability by methyl thiazolyl tetrazolium (MTT) assay. We also determined membrane integrity and apoptosis of A549 cells after different doses of TiO 2 /GSs exposure by LDH assay and flow cytometry. Assay of A549 cell viability showed regular reductions with a time-and dose-dependent tendency after exposure to pure TiO 2 , TiO 2 /4.2wt% GSs and TiO 2 /6wt% GSs for 24 hrs or 48 hrs. The LDH released and cellular apoptosis also had a dose-dependent effect, which was associated with the surface area of TiO 2 /4.2wt% GSs. Our results provide essential knowledge of the acceptable biocompatibility of TiO 2 /GSs nanocomposites, and only when cells were exposed at a high concentration (≥50 μg/mL), and for a prolonged period of time did TiO 2 /GSs nanoparticles exhibit minimal cytotoxicity against A549 cells." @default.
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• W2000335112 date "2014-10-01" @default.
• W2000335112 modified "2023-09-24" @default.
• W2000335112 title "<i>In Vitro</i> Cytotoxic Studies of Ultrafine TiO₂/Graphene Oxide Sheet Nanocomposites in Human Alveolar Epithelial Cells" @default.
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• W2000335112 doi "https://doi.org/10.4028/www.scientific.net/amr.1051.353" @default.
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