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• W2000338345 endingPage "680" @default.
• W2000338345 startingPage "673" @default.
• W2000338345 abstract "Increased levels of nitric oxide (NO) at an inflammatory site may affect the biological activity of lymphoid cells. To investigate the effects of NO on the immune system, we measured the mitochondrial membrane potential (delta psi m) of the peripheral blood lymphocytes (PBL) cultured with a chemical NO donor. PBL from healthy volunteers were cultured with NOC18, a NO-generating compound, at various concentrations. The delta psi m of the PBL was measured by flow-cytometry using 3,3-dihexyloxacarbocyanine iodide (DiOC6(3)). NOC18 induced a decrease in the delta psi m of the PBL in a dose-dependent fashion, induced an increase in the levels of reactive oxygen species (ROS), and caused these cells to undergo apoptosis. Dual-color staining of the delta psi m and lymphocyte surface markers demonstrated that CD3-CD56+ natural killer (NK) cells were responsive to NO. Trolox, a vitamin E analog, partially reversed the NO-induced decrease in the delta psi m of the PBL. We showed that the delta psi m of peripheral NK cells were decreased by NO, which suggests that abundant NO at an inflammatory site may impair NK cell function." @default.
• W2000338345 created "2016-06-24" @default.
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• W2000338345 date "2000-12-01" @default.
• W2000338345 modified "2023-09-26" @default.
• W2000338345 title "Nitric Oxide Induces a Decrease in the Mitochondrial Membrane Potential of Peripheral Blood Lymphocytes, Especially in Natural Killer Cells" @default.
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• W2000338345 doi "https://doi.org/10.1089/ars.2000.2.4-673" @default.
• W2000338345 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11213472" @default.
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