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• W2000338638 endingPage "1143" @default.
• W2000338638 startingPage "1133" @default.
• W2000338638 abstract "Summary Wetlands, including peatlands, are the main source of natural methane emission. Well‐defined fen microsites have different methane emissions rates, but it is not known whether the methane‐producing Archaea communities vary at these sites. Possible horizontal variations of communities, in a natural oligotrophic fen, were analysed by characterizing the methanogens from two well‐defined microsites: Eriophorum lawn and Hummock. Community structures were studied at two different layers of the fen, showing, respectively, high and low methane production. The structure of methanogen populations was determined using molecular techniques targeting the 16SrRNA gene and combined denaturing gradient gel electrophoresis (DGGE) and restriction fragment length polymorphism (RFLP) analysis. Results subjected to non‐metric multidimensional scaling (MDS), diversity indices calculation and phylogenetic analysis revealed that upper layer communities changed with site while deeper layer communities remained the same. Phylogenetic analyses revealed six different clusters of sequences grouping with only two known orders of methanogens. Upper layers of Hummock were dominated by sequences clustering with members of Methanomicrobiales and sequences dominating the upper part of the Eriophorum lawn were related to members of the order Methanosarcinales . Novel methanogenic sequences were found at both sites at both depths. Vegetation characterizing the microsites probably influences the microbial communities in the layers of the fen where methane is produced." @default.
• W2000338638 created "2016-06-24" @default.
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• W2000338638 creator A5039889152 @default.
• W2000338638 creator A5086887956 @default.
• W2000338638 date "2003-10-16" @default.
• W2000338638 modified "2023-10-16" @default.
• W2000338638 title "Microsite-dependent changes in methanogenic populations in a boreal oligotrophic fen" @default.
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• W2000338638 doi "https://doi.org/10.1046/j.1462-2920.2003.00520.x" @default.
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