Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000339963> ?p ?o ?g. }
- W2000339963 endingPage "2585" @default.
- W2000339963 startingPage "2574" @default.
- W2000339963 abstract "We report the first structural analysis of an integral membrane protein of the bacterial divisome. FtsB is a single-pass membrane protein with a periplasmic coiled coil. Its heterologous association with its partner FtsL represents an essential event for the recruitment of the late components to the division site. Using a combination of mutagenesis, computational modeling, and X-ray crystallography, we determined that FtsB self-associates, and we investigated its structural organization. We found that the transmembrane domain of FtsB homo-oligomerizes through an evolutionarily conserved interaction interface where a polar residue (Gln 16) plays a critical role through the formation of an interhelical hydrogen bond. The crystal structure of the periplasmic domain, solved as a fusion with Gp7, shows that 30 juxta-membrane amino acids of FtsB form a canonical coiled coil. The presence of conserved Gly residue in the linker region suggests that flexibility between the transmembrane and coiled coil domains is functionally important. We hypothesize that the transmembrane helices of FtsB form a stable dimeric core for its association with FtsL into a higher-order oligomer and that FtsL is required to stabilize the periplasmic domain of FtsB, leading to the formation of a complex that is competent for binding to FtsQ, and to their consequent recruitment to the divisome. The study provides an experimentally validated structural model and identifies point mutations that disrupt association, thereby establishing important groundwork for the functional characterization of FtsB in vivo." @default.
- W2000339963 created "2016-06-24" @default.
- W2000339963 creator A5003047324 @default.
- W2000339963 creator A5008115009 @default.
- W2000339963 creator A5008836580 @default.
- W2000339963 creator A5011602419 @default.
- W2000339963 creator A5014832561 @default.
- W2000339963 creator A5054271621 @default.
- W2000339963 date "2013-04-04" @default.
- W2000339963 modified "2023-09-25" @default.
- W2000339963 title "Structural Organization of FtsB, a Transmembrane Protein of the Bacterial Divisome" @default.
- W2000339963 cites W1510774549 @default.
- W2000339963 cites W1539796472 @default.
- W2000339963 cites W1604452613 @default.
- W2000339963 cites W1608724452 @default.
- W2000339963 cites W1829689588 @default.
- W2000339963 cites W1885686633 @default.
- W2000339963 cites W1919928164 @default.
- W2000339963 cites W1959649751 @default.
- W2000339963 cites W1965259129 @default.
- W2000339963 cites W1965431973 @default.
- W2000339963 cites W1967446612 @default.
- W2000339963 cites W1969787762 @default.
- W2000339963 cites W1973390135 @default.
- W2000339963 cites W1977896619 @default.
- W2000339963 cites W197862321 @default.
- W2000339963 cites W1981942699 @default.
- W2000339963 cites W1984711666 @default.
- W2000339963 cites W1990737987 @default.
- W2000339963 cites W1998361708 @default.
- W2000339963 cites W1999590520 @default.
- W2000339963 cites W2000214816 @default.
- W2000339963 cites W2001290027 @default.
- W2000339963 cites W2002718534 @default.
- W2000339963 cites W2008556223 @default.
- W2000339963 cites W2009763277 @default.
- W2000339963 cites W2011963149 @default.
- W2000339963 cites W2013881981 @default.
- W2000339963 cites W2015381530 @default.
- W2000339963 cites W2018982114 @default.
- W2000339963 cites W2022796341 @default.
- W2000339963 cites W2026580217 @default.
- W2000339963 cites W2028843689 @default.
- W2000339963 cites W2033323125 @default.
- W2000339963 cites W2033642378 @default.
- W2000339963 cites W2035873802 @default.
- W2000339963 cites W2036158497 @default.
- W2000339963 cites W2037846060 @default.
- W2000339963 cites W2041256732 @default.
- W2000339963 cites W2041970862 @default.
- W2000339963 cites W2044217069 @default.
- W2000339963 cites W2052800057 @default.
- W2000339963 cites W2053271137 @default.
- W2000339963 cites W2053304303 @default.
- W2000339963 cites W2055043387 @default.
- W2000339963 cites W2058858340 @default.
- W2000339963 cites W2062016903 @default.
- W2000339963 cites W2067554217 @default.
- W2000339963 cites W2077605949 @default.
- W2000339963 cites W2078652488 @default.
- W2000339963 cites W2082245646 @default.
- W2000339963 cites W2085748469 @default.
- W2000339963 cites W2087098118 @default.
- W2000339963 cites W2095750018 @default.
- W2000339963 cites W2097459210 @default.
- W2000339963 cites W2110299776 @default.
- W2000339963 cites W2111198021 @default.
- W2000339963 cites W2111482169 @default.
- W2000339963 cites W2112612730 @default.
- W2000339963 cites W2123686853 @default.
- W2000339963 cites W2126211773 @default.
- W2000339963 cites W2127494319 @default.
- W2000339963 cites W2128428835 @default.
- W2000339963 cites W2135807487 @default.
- W2000339963 cites W2143117974 @default.
- W2000339963 cites W2143632667 @default.
- W2000339963 cites W2144081223 @default.
- W2000339963 cites W2144743370 @default.
- W2000339963 cites W2145917285 @default.
- W2000339963 cites W2146987229 @default.
- W2000339963 cites W2150010294 @default.
- W2000339963 cites W2158522775 @default.
- W2000339963 cites W2158714788 @default.
- W2000339963 cites W2163070730 @default.
- W2000339963 cites W2165499430 @default.
- W2000339963 cites W2165531707 @default.
- W2000339963 cites W2166676244 @default.
- W2000339963 cites W2168777174 @default.
- W2000339963 cites W2170719146 @default.
- W2000339963 doi "https://doi.org/10.1021/bi400222r" @default.
- W2000339963 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3702382" @default.
- W2000339963 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23520975" @default.
- W2000339963 hasPublicationYear "2013" @default.
- W2000339963 type Work @default.
- W2000339963 sameAs 2000339963 @default.
- W2000339963 citedByCount "35" @default.
- W2000339963 countsByYear W20003399632013 @default.
- W2000339963 countsByYear W20003399632014 @default.