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- W2000340413 abstract "Cerebral ischemia is encompassed by cerebrovascular apoptosis, yet the mechanisms behind apoptosis regulation are not fully understood. We previously demonstrated inhibition of endothelial apoptosis by vascular endothelial growth factor (VEGF) through upregulation of poly(ADP-ribose)-polymerase (PARP) expression. However, PARP overactivation through oxidative stress can lead to necrosis. This study tested the hypothesis that neuropilin-1 (NP-1), an alternative VEGF receptor, regulates the response to cerebral ischemia by modulating PARP expression and, in turn, apoptosis inhibition by VEGF. In endothelial cell culture, NP-1 colocalized with VEGF receptor-2 (VEGFR-2) and acted as its coreceptor. This significantly enhanced VEGF-induced PARP mRNA and protein expression demonstrated by receptor-specific inhibitors and VEGF-A isoforms. NP-1 augmented the inhibitory effect of VEGF/VEGFR-2 interaction on apoptosis induced by adhesion inhibition through the αV-integrin inhibitor cRGDfV. NP-1/VEGFR-2 signal transduction involved JNK and Akt. In rat models of permanent and temporary middle cerebral artery occlusion, the ischemic cerebral hemispheres displayed endothelial and neuronal apoptosis next to increased endothelial NP-1 and VEGFR-2 expression compared to non-ischemic cerebral hemispheres, sham-operated or untreated controls. Increased vascular superoxide dismutase-1 and catalase expression as well as decreased glycogen reserves indicated oxidative stress in the ischemic brain. Of note, protein levels of intact PARP remained stable despite pro-apoptotic conditions through increased PARP mRNA production during cerebral ischemia. In conclusion, NP-1 is upregulated in conditions of imminent cerebrovascular apoptosis to reinforce apoptosis inhibition and modulate VEGF-dependent PARP expression and activation. We propose that NP-1 is a key modulator of VEGF maintaining cerebrovascular integrity during ischemia. Modulating the function of NP-1 to target PARP could help to prevent cellular damage in cerebrovascular disease." @default.
- W2000340413 created "2016-06-24" @default.
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- W2000340413 date "2013-11-01" @default.
- W2000340413 modified "2023-09-24" @default.
- W2000340413 title "Neuropilin-1 modulates vascular endothelial growth factor-induced poly(ADP-ribose)-polymerase leading to reduced cerebrovascular apoptosis" @default.
- W2000340413 cites W1492224794 @default.
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- W2000340413 cites W1949006326 @default.
- W2000340413 cites W1964621218 @default.
- W2000340413 cites W1965119464 @default.
- W2000340413 cites W1966259557 @default.
- W2000340413 cites W1966293423 @default.
- W2000340413 cites W1969113805 @default.
- W2000340413 cites W1984759940 @default.
- W2000340413 cites W1986563371 @default.
- W2000340413 cites W1987454669 @default.
- W2000340413 cites W1987868279 @default.
- W2000340413 cites W1989658253 @default.
- W2000340413 cites W1991463736 @default.
- W2000340413 cites W1995840246 @default.
- W2000340413 cites W1997300044 @default.
- W2000340413 cites W1998584272 @default.
- W2000340413 cites W2000771735 @default.
- W2000340413 cites W2003240567 @default.
- W2000340413 cites W2007391140 @default.
- W2000340413 cites W2010641085 @default.
- W2000340413 cites W2011403734 @default.
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- W2000340413 doi "https://doi.org/10.1016/j.nbd.2013.06.009" @default.
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