FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000346045> ?p ?o ?g. }

• W2000346045 endingPage "191" @default.
• W2000346045 startingPage "182" @default.
• W2000346045 abstract "Abstract A simple dissociation procedure and the collagen gel culture system have been utilized to determine the effects of mammogenic hormones and epidermal growth factor (EGF) on the proliferation of normal rat mammary epithelial (RME) cells in serum‐free culture. Epithelial fragments, isolated from normal virgin F344 rat mammary glands by enzyme digestion followed by Percoll density gradient centrifugation, were embedded within a rat tail collagen matrix. A three‐to four‐fold increase in cell number was observed when ovine prolactin (PRL) and progesterone (P) were present in the basal medium during 7 days of culture. Mouse EGF stimulated one cell doubling during the same culture period. Isolated mammary organoids produced a ‘stellate’ type colony when PRL + P were present in the culture medium. These colonies were composed of small, tightly packed cuboidal cells. The addition of EGF to the basal medium produced a diffuse ‘basket’ type colony which was composed of large, elongate cells. When the complete hormonal and growth factor combination (PRL + P + EGF) was present, a ‘mixed’ type colony was observed which contained both the large and small epithelial cell types. Immunocytochemical analysis revealed that both the cuboidal and elongate cells present in the two colony types stained with antibodies to keratin indicating that these cells were epithelial in nature. The small cuboidal cells also expressed thioesterase II and alpha‐lactalbumin, both specific for secretory mammary epithelial cells. The large, elongate cell type, however, was positive for actin but did not stain for either secretory epithelial specific marker. The results reported here suggest that normal rat mammary tissue may contain two epithelial populations, one which responds to PRL + P and the other which responds to EGF." @default.
• W2000346045 created "2016-06-24" @default.
• W2000346045 creator A5061317895 @default.
• W2000346045 creator A5082310426 @default.
• W2000346045 creator A5083497493 @default.
• W2000346045 date "1985-11-01" @default.
• W2000346045 modified "2023-10-16" @default.
• W2000346045 title "Differential response of normal rat mammary epithelial cells to mammogenic hormones and EGF" @default.
• W2000346045 cites W1463893905 @default.
• W2000346045 cites W1497579369 @default.
• W2000346045 cites W1516227994 @default.
• W2000346045 cites W1565526431 @default.
• W2000346045 cites W1567780941 @default.
• W2000346045 cites W1594165376 @default.
• W2000346045 cites W1964736779 @default.
• W2000346045 cites W1970349987 @default.
• W2000346045 cites W1981305966 @default.
• W2000346045 cites W1992460852 @default.
• W2000346045 cites W1994348629 @default.
• W2000346045 cites W2021144709 @default.
• W2000346045 cites W2032552530 @default.
• W2000346045 cites W2040720266 @default.
• W2000346045 cites W2052043489 @default.
• W2000346045 cites W2060183661 @default.
• W2000346045 cites W2064449411 @default.
• W2000346045 cites W2069445428 @default.
• W2000346045 cites W2069503254 @default.
• W2000346045 cites W2078687852 @default.
• W2000346045 cites W2086013495 @default.
• W2000346045 cites W2086047876 @default.
• W2000346045 cites W2094915902 @default.
• W2000346045 cites W2101517199 @default.
• W2000346045 cites W2108640628 @default.
• W2000346045 cites W2111486924 @default.
• W2000346045 cites W2112219947 @default.
• W2000346045 cites W2170423056 @default.
• W2000346045 cites W2258025255 @default.
• W2000346045 cites W2802463337 @default.
• W2000346045 doi "https://doi.org/10.1002/jcp.1041250203" @default.
• W2000346045 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3877058" @default.
• W2000346045 hasPublicationYear "1985" @default.
• W2000346045 type Work @default.
• W2000346045 sameAs 2000346045 @default.
• W2000346045 citedByCount "55" @default.
• W2000346045 countsByYear W20003460452013 @default.
• W2000346045 countsByYear W20003460452015 @default.
• W2000346045 countsByYear W20003460452016 @default.
• W2000346045 crossrefType "journal-article" @default.
• W2000346045 hasAuthorship W2000346045A5061317895 @default.
• W2000346045 hasAuthorship W2000346045A5082310426 @default.
• W2000346045 hasAuthorship W2000346045A5083497493 @default.
• W2000346045 hasConcept C126322002 @default.
• W2000346045 hasConcept C132946820 @default.
• W2000346045 hasConcept C134018914 @default.
• W2000346045 hasConcept C1491633281 @default.
• W2000346045 hasConcept C151730666 @default.
• W2000346045 hasConcept C153911025 @default.
• W2000346045 hasConcept C189014844 @default.
• W2000346045 hasConcept C202751555 @default.
• W2000346045 hasConcept C2776362946 @default.
• W2000346045 hasConcept C2779064019 @default.
• W2000346045 hasConcept C2908830777 @default.
• W2000346045 hasConcept C48679268 @default.
• W2000346045 hasConcept C529295009 @default.
• W2000346045 hasConcept C54355233 @default.
• W2000346045 hasConcept C55493867 @default.
• W2000346045 hasConcept C71315377 @default.
• W2000346045 hasConcept C71924100 @default.
• W2000346045 hasConcept C81241287 @default.
• W2000346045 hasConcept C81885089 @default.
• W2000346045 hasConcept C86803240 @default.
• W2000346045 hasConcept C98648569 @default.
• W2000346045 hasConceptScore W2000346045C126322002 @default.
• W2000346045 hasConceptScore W2000346045C132946820 @default.
• W2000346045 hasConceptScore W2000346045C134018914 @default.
• W2000346045 hasConceptScore W2000346045C1491633281 @default.
• W2000346045 hasConceptScore W2000346045C151730666 @default.
• W2000346045 hasConceptScore W2000346045C153911025 @default.
• W2000346045 hasConceptScore W2000346045C189014844 @default.
• W2000346045 hasConceptScore W2000346045C202751555 @default.
• W2000346045 hasConceptScore W2000346045C2776362946 @default.
• W2000346045 hasConceptScore W2000346045C2779064019 @default.
• W2000346045 hasConceptScore W2000346045C2908830777 @default.
• W2000346045 hasConceptScore W2000346045C48679268 @default.
• W2000346045 hasConceptScore W2000346045C529295009 @default.
• W2000346045 hasConceptScore W2000346045C54355233 @default.
• W2000346045 hasConceptScore W2000346045C55493867 @default.
• W2000346045 hasConceptScore W2000346045C71315377 @default.
• W2000346045 hasConceptScore W2000346045C71924100 @default.
• W2000346045 hasConceptScore W2000346045C81241287 @default.
• W2000346045 hasConceptScore W2000346045C81885089 @default.
• W2000346045 hasConceptScore W2000346045C86803240 @default.
• W2000346045 hasConceptScore W2000346045C98648569 @default.
• W2000346045 hasIssue "2" @default.
• W2000346045 hasLocation W20003460451 @default.
• W2000346045 hasLocation W20003460452 @default.
• W2000346045 hasOpenAccess W2000346045 @default.
• W2000346045 hasPrimaryLocation W20003460451 @default.

• next