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• W2000352671 startingPage "7402" @default.
• W2000352671 abstract "The group I self-splicing introns act at exon-intron junctions without recognizing a particular sequence. In order to understand splice-site selection, we have developed an assay system based on the Tetrahymena ribozyme to allow the study of numerous 5'-splice-site variants. Cleavage at the correct site requires formation of the correct secondary structure and occurs most efficiently within a 3-base-pair window centered on base pair 5 from the bottom of the P1 stem. Within this window the ribozyme recognizes and cleaves at a wobble base pair; the base pair above the cleavage site also influences splicing efficiency. The recognition of RNA structure rather than sequence explains the ability of these transposable introns to splice out of a variety of sequence contexts." @default.
• W2000352671 created "2016-06-24" @default.
• W2000352671 creator A5023274897 @default.
• W2000352671 creator A5062189398 @default.
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• W2000352671 date "1989-10-01" @default.
• W2000352671 modified "2023-10-13" @default.
• W2000352671 title "RNA structure, not sequence, determines the 5' splice-site specificity of a group I intron." @default.
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• W2000352671 doi "https://doi.org/10.1073/pnas.86.19.7402" @default.
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