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- W2000355901 abstract "To facilitate improved diagnosis and detection of the third stage larva (L3) of Anisakis pegreffii from the Minnan-Taiwan bank fishing ground in Taiwan Strait, a real-time PCR method for the detection in situ and differentiation was developed to amplify a region of the second internal transcribed spacer (ITS-2) of this parasite. The real-time PCR assay was capable of detecting 1/3 of a single L3 in 30 mg of marine fish tissue, and also exhibited a high level of specificity for A. pegreffii, no fluorescence signals were observed in other five major larval anisakid species found in commercial marine fishes caught in this fishing ground." @default.
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- W2000355901 date "2011-02-01" @default.
- W2000355901 modified "2023-09-23" @default.
- W2000355901 title "Anisakis pegreffii: A quantitative fluorescence PCR assay for detection in situ" @default.
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- W2000355901 doi "https://doi.org/10.1016/j.exppara.2010.11.008" @default.
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