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- W2000356445 abstract "A method for determining the concentration-dependent mutual diffusion coefficient D(C) of a macromolecule-solvent combination over a wide macromolecular concentration range is presented. All necessary data are gathered from a single experiment, in which polymer concentration profiles are measured during one-dimensional dead-end ultrafiltration. Based on these profiles, the convection-diffusion equation is used to deduce the dependence of D on C. To demonstrate the utility of the approach, studies using sodium hyaluronate dissolved in either 10 mM NaCl or a phosphate buffer were carried out. For hyaluronate (HA) in 10 mM NaCl, the mutual diffusion coefficient varies approximately linearly with concentration according to D(C) = 4.1 × 10−6 C0.96 cm2/s in the range 0 ≤ C ≤ 0.6 mass %, for C expressed in mass %. However, transition points (slope changes) in the D(C) curve are present at C ≅ 0.7 mass % and C ≅ 1.4 mass %. For HA in the phosphate buffer, the mutual diffusion coefficient is well described by D(C) = 1.9 × 10−6 C0.825, for 0 ≤ C ≤ 1.8 mass %. These values agree well with previously published data. The technique is robust, and permits reasonably high polymer concentrations to be easily studied. © 1996 John Wiley & Sons, Inc." @default.
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- W2000356445 date "1996-07-01" @default.
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- W2000356445 title "Obtaining the concentration-dependent diffusion coefficient from ultrafiltration experiments: Application to hyaluronate" @default.
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- W2000356445 doi "https://doi.org/10.1002/(sici)1097-0282(199607)39:1<1::aid-bip1>3.0.co;2-u" @default.
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