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• W2000357748 abstract "A high-performance liquid chromatographic method has been developed for the determination of 4-hydroxyifosfamide, a metabolite of ifosfamide, in plasma of cancer patients. The analyte is derivatized to 7-hydroxyquinoline, which can be detached fluorimetrically. The calibration graph is linear in the concentration range 0.05-25 microM, the limit of detection being 40 nM. Any inference from acrolein, another metabolite of ifosfamide, was ruled out. 4-Hydroxyifosfamide is very unstable in plasma and a stabilization procedure by adding citric acid has been developed. Thus treated, the samples were stable for 4 days. Analysis of a patient's plasma samples revealed that the 4-hydroxifosfamide concentration did not exceed 10 microM." @default.
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• W2000357748 date "1997-03-01" @default.
• W2000357748 modified "2023-10-14" @default.
• W2000357748 title "Determination of 4-hydroxyifosfamide in biological matrices by high-performance liquid chromatography" @default.
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• W2000357748 doi "https://doi.org/10.1016/s0731-7085(96)01893-6" @default.
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