Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000359966> ?p ?o ?g. }
- W2000359966 endingPage "148" @default.
- W2000359966 startingPage "135" @default.
- W2000359966 abstract "Transglutaminase (TG) enzymes and protein crosslinking have long been implicated in the formation of mineralized tissues. The aim of this study was to analyze the expression, activity and function of TGs in differentiating osteoblasts to gain further insight into the role of extracellular matrix protein crosslinking in bone formation. MC3T3-E1 (subclone 14) pre-osteoblast cultures were treated with ascorbic acid and β-glycerophosphate to induce cell differentiation and matrix mineralization. Expression of TG isoforms was analyzed by RT-PCR. TG activity was assessed during osteoblast differentiation by in vitro biochemical assays and by in situ labeling of live cell cultures. We demonstrate that MC3T3-E1/C14 osteoblasts express two TG isoforms—TG2 and FXIIIA. Abundant TG activity was observed during cell differentiation which increased significantly after thrombin treatment, a result confirming the presence of FXIIIA in the cultures. Ascorbic acid treatment, which stimulated collagen secretion and assembly, also stimulated externalization of TG activity, likely from FXIIIA which was externalized upon this treatment as analyzed by immunofluoresence microscopy. Inhibition of TG activity in the cultures by cystamine resulted in complete abrogation of mineralization, attributable to decreased matrix accumulation and an arrested state of osteoblast differentiation as measured by decreased levels of bone sialoprotein, osteocalcin and alkaline phosphatase. Additional functional studies and substrate characterization showed that TG activity was required for the formation of a fibronectin–collagen network during the early stages of matrix formation and assembly. This network, in turn, appeared to be essential for further matrix production and progression of the osteoblast differentiation program, and ultimately for mineralization." @default.
- W2000359966 created "2016-06-24" @default.
- W2000359966 creator A5028300109 @default.
- W2000359966 creator A5034380430 @default.
- W2000359966 creator A5041657079 @default.
- W2000359966 creator A5045826335 @default.
- W2000359966 creator A5066996132 @default.
- W2000359966 creator A5085202255 @default.
- W2000359966 date "2006-04-01" @default.
- W2000359966 modified "2023-10-05" @default.
- W2000359966 title "Transglutaminase activity regulates osteoblast differentiation and matrix mineralization in MC3T3-E1 osteoblast cultures" @default.
- W2000359966 cites W122132793 @default.
- W2000359966 cites W1590619267 @default.
- W2000359966 cites W1909072940 @default.
- W2000359966 cites W1967455040 @default.
- W2000359966 cites W1970567588 @default.
- W2000359966 cites W1972775413 @default.
- W2000359966 cites W1979164076 @default.
- W2000359966 cites W1979742792 @default.
- W2000359966 cites W1980213051 @default.
- W2000359966 cites W1986366555 @default.
- W2000359966 cites W1989962141 @default.
- W2000359966 cites W1996577769 @default.
- W2000359966 cites W1997649080 @default.
- W2000359966 cites W1998889604 @default.
- W2000359966 cites W1998971716 @default.
- W2000359966 cites W1999669871 @default.
- W2000359966 cites W2000201832 @default.
- W2000359966 cites W2000962915 @default.
- W2000359966 cites W2005248263 @default.
- W2000359966 cites W2007046840 @default.
- W2000359966 cites W2007421109 @default.
- W2000359966 cites W2009190820 @default.
- W2000359966 cites W2010528049 @default.
- W2000359966 cites W2014097308 @default.
- W2000359966 cites W2017405539 @default.
- W2000359966 cites W2018081553 @default.
- W2000359966 cites W2022356764 @default.
- W2000359966 cites W2027264961 @default.
- W2000359966 cites W2028457075 @default.
- W2000359966 cites W2028788241 @default.
- W2000359966 cites W2029551351 @default.
- W2000359966 cites W2035978536 @default.
- W2000359966 cites W2036603824 @default.
- W2000359966 cites W2036806537 @default.
- W2000359966 cites W2043702753 @default.
- W2000359966 cites W2044980091 @default.
- W2000359966 cites W2046359243 @default.
- W2000359966 cites W2057918576 @default.
- W2000359966 cites W2060016638 @default.
- W2000359966 cites W2060115924 @default.
- W2000359966 cites W2066416451 @default.
- W2000359966 cites W2075108741 @default.
- W2000359966 cites W2081232429 @default.
- W2000359966 cites W2084028904 @default.
- W2000359966 cites W2084360725 @default.
- W2000359966 cites W2086710992 @default.
- W2000359966 cites W2093966629 @default.
- W2000359966 cites W2095214536 @default.
- W2000359966 cites W2108118435 @default.
- W2000359966 cites W2120498307 @default.
- W2000359966 cites W2122358120 @default.
- W2000359966 cites W2127972419 @default.
- W2000359966 cites W2129611079 @default.
- W2000359966 cites W2135334875 @default.
- W2000359966 cites W2144272048 @default.
- W2000359966 cites W2147339154 @default.
- W2000359966 cites W2151982166 @default.
- W2000359966 cites W2169170480 @default.
- W2000359966 cites W2170608174 @default.
- W2000359966 cites W2184338168 @default.
- W2000359966 cites W2404907151 @default.
- W2000359966 cites W4252724004 @default.
- W2000359966 doi "https://doi.org/10.1016/j.matbio.2005.11.001" @default.
- W2000359966 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16469487" @default.
- W2000359966 hasPublicationYear "2006" @default.
- W2000359966 type Work @default.
- W2000359966 sameAs 2000359966 @default.
- W2000359966 citedByCount "107" @default.
- W2000359966 countsByYear W20003599662012 @default.
- W2000359966 countsByYear W20003599662013 @default.
- W2000359966 countsByYear W20003599662014 @default.
- W2000359966 countsByYear W20003599662015 @default.
- W2000359966 countsByYear W20003599662016 @default.
- W2000359966 countsByYear W20003599662017 @default.
- W2000359966 countsByYear W20003599662018 @default.
- W2000359966 countsByYear W20003599662019 @default.
- W2000359966 countsByYear W20003599662020 @default.
- W2000359966 countsByYear W20003599662021 @default.
- W2000359966 countsByYear W20003599662022 @default.
- W2000359966 countsByYear W20003599662023 @default.
- W2000359966 crossrefType "journal-article" @default.
- W2000359966 hasAuthorship W2000359966A5028300109 @default.
- W2000359966 hasAuthorship W2000359966A5034380430 @default.
- W2000359966 hasAuthorship W2000359966A5041657079 @default.
- W2000359966 hasAuthorship W2000359966A5045826335 @default.
- W2000359966 hasAuthorship W2000359966A5066996132 @default.
- W2000359966 hasAuthorship W2000359966A5085202255 @default.