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- W2000365788 abstract "RATIONALE: The interaction between IgE, allergen and CD23 may be the primary mechanism behind activation of allergen-specific T-cells through facilitated antigen presentation (FAP) at low allergen concentration in vivo. Here we show how differences in IgE antibody-affinity influence FAP-induced T-cell activation and how the phenotype of antigen presenting cells (APC) is affected by CD23 mediated allergen uptake. METHODS: Complex-formation between IgE (Derp2-specific rIgE or human serum), Derp2 and CD23 on CD23+ B-cells was measured by flow cytometry. FAP-mediated T-cell activation was measured in parallel using the complex-bearing B-cells as APC for stimulation of Derp2-specific T-cell lines. T-cell proliferation was investigated in thymidin incorporation assay at various time-points with or without fixation of the APC. APC expression of MHCII, CD23, CD32, CD80, and CD86 was determined by flow cytometry. RESULTS: The efficiency of both B-cell complex binding and T-cell activation increased with increasing IgE affinity irrespective of the duration of complex-internalization (30-240 min.), while B-cell binding of IgE-allergen complexes was enhanced at 0°C when compared to 37°C. The time from complex binding/uptake to presentation on the APC surface was between 6 and 16 hours and within this timeframe complex internalization/CD23 cross-linking did not induce changes in APC expression of surface molecules. CONCLUSIONS: Increasing IgE affinity leads to increases in antigen uptake and subsequent allergen-specific T-cell activation. IgE-affinity and not internalization time determines the level of FAP induced T-cell activation. Complex-binding to APC at 37°C did more closely reflect the subsequent T-cell activation and CD23 cross-linking did not affect APC phenotype." @default.
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- W2000365788 date "2010-02-01" @default.
- W2000365788 modified "2023-09-26" @default.
- W2000365788 title "IgE Affinity Dictates Allergen Binding To Cd23+ B-cells And Subsequent T-cell Activation Through Facilitated Antigen Presentation" @default.
- W2000365788 doi "https://doi.org/10.1016/j.jaci.2009.12.891" @default.
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