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• W2000367672 abstract "The bronze ( bz ) locus of maize has been cloned by an indirect procedure utilizing the cloned transposable controlling element Activator ( Ac ). Restriction endonuclease fragments of maize DNA were cloned in bacteriophage λ and recombinant phage with homology to the center of the Ac element were isolated. The cloned fragments were analyzed to determine which contained sequences that were structurally identical to a previously isolated Ac element. Two such fragments were identified. Sequences flanking the Ac element were subcloned and used to probe genomic DNA from plants with well-defined mutations at the bz locus. By this means, it was established that one of the genomic clones contained a bz locus sequence. The subcloned probe fragment was then used to clone a nonmutant Bz allele of the locus. The method described here should prove useful in cloning other loci with Ac insertion mutations." @default.
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• W2000367672 date "1984-06-01" @default.
• W2000367672 modified "2023-10-05" @default.
• W2000367672 title "Cloning of the <i>bronze</i> locus in maize by a simple and generalizable procedure using the transposable controlling element <i>Activator</i> ( <i>Ac</i> )" @default.
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• W2000367672 doi "https://doi.org/10.1073/pnas.81.12.3825" @default.
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