FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000367706> ?p ?o ?g. }

• W2000367706 abstract "Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, ILPurpose: Curcumin appears to be a promising chemopreventive agent for various human cancers including breast cancer (BC). However, the molecular mechanism(s) by which it functions in vivo have not been fully elucidated. Recently, we have shown curcumin to be an effective hypomethylating agent in acute myeloid leukemia cells. Here, we investigated the ability of curcumin and its major metabolite, curcumin O-glucuronide (COG) to epigenetically modulate tumor suppressor gene expression in BC cells. Method: MCF-7 and MDA-MB-231 BC cells were treated with curcumin and COG (0.1 to 20 μM) for up to 96 hrs. MCF-7 and MDA-MB-231 cell engrafted tumor-bearing nude mice were treated with i.p. doses of 100 mg/kg curcumin or oral doses of 300 mg/kg nanoemulsion curcumin (NEC). The mRNA levels of RASSF1A, TIMP-3, DNMT1, microRNA29b, and microRNA221/222 were assessed by RT-PCR and protein levels were measured by Western Blot. DNA methylation levels were assessed using LC-MS. The in vitro anti-proliferative activities of curcumin, COG and NEC and the in vivo anti-tumor growth activities of curcumin and NEC were evaluated. Results: Curcumin induced significant global DNA hypomethylation in MCF-7 cells and reactivated RASSF1A expression in both MCF-7 and MDA-MB-231 cells. In vivo reactivation of RASSF1A by curcumin was confirmed in MCF-7 engrafted tumor tissues in mice. Curcumin reactivated TIMP-3 expression in MCF-7 engrafted tumor tissues, but down-regulated TIMP-3 expression in MCF-7 cells in vitro. We have evidence that this discrepancy was due to differential metabolism of curcumin to COG in vitro (no COG formation) and in vivo (COG level > curcumin), since COG itself reactivated TIMP-3 in MCF-7 cells. Notably, COG down-regulated microRNA221/222, two oncogenic microRNAs that suppress TIMP-3 expression, which may account for TIMP-3 reactivation in MCF-7 cells by COG. Importantly, COG chemically inhibited DNMT1 methylation activity and up-regulated microRNA29b, a central regulator of DNMTs in MCF-7 and MDA-MB-231 cells, which may be responsible for its hypomethylating activity. Curcumin elicited anti-tumor growth effects in both MCF-7 (70%) and MDA-MB-231 (27%) cell engrafted tumor bearing nude mice after four weeks of daily i.p. injection. Notably, NEC inhibited MDA-MB-231 cell engrafted tumor growth up to 50%, nearly twice that of curcumin. Conclusion: Curcumin reactivated RASSF1A and TIMP-3 tumor suppressor genes in vivo presumably through its conversion to COG and COG's modulation of DNA methylation and microRNAs. NEC is a more potent formulation for MDA-MB-231 cell engrafted tumor growth in nude mice when compared to curcumin. Further evaluation of NEC for BC prevention or treatment either alone or in combination with clinically-used agents is warranted. Supported by R21 CA135478 [ZL] and biomedical mass spectrometry laboratory (KKC and ZL).Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 581. doi:1538-7445.AM2012-581" @default.
• W2000367706 created "2016-06-24" @default.
• W2000367706 creator A5012262072 @default.
• W2000367706 creator A5015153331 @default.
• W2000367706 creator A5017649557 @default.
• W2000367706 creator A5030823966 @default.
• W2000367706 creator A5032442574 @default.
• W2000367706 creator A5052947767 @default.
• W2000367706 creator A5056111187 @default.
• W2000367706 creator A5062250759 @default.
• W2000367706 creator A5064955096 @default.
• W2000367706 creator A5084771066 @default.
• W2000367706 creator A5086348397 @default.
• W2000367706 date "2012-04-15" @default.
• W2000367706 modified "2023-09-25" @default.
• W2000367706 title "Abstract 581: Complementary reactivation of tumor suppressor genes in breast cancer cells by curcumin and curcumin O-glucuronide" @default.
• W2000367706 doi "https://doi.org/10.1158/1538-7445.am2012-581" @default.
• W2000367706 hasPublicationYear "2012" @default.
• W2000367706 type Work @default.
• W2000367706 sameAs 2000367706 @default.
• W2000367706 citedByCount "0" @default.
• W2000367706 crossrefType "proceedings-article" @default.
• W2000367706 hasAuthorship W2000367706A5012262072 @default.
• W2000367706 hasAuthorship W2000367706A5015153331 @default.
• W2000367706 hasAuthorship W2000367706A5017649557 @default.
• W2000367706 hasAuthorship W2000367706A5030823966 @default.
• W2000367706 hasAuthorship W2000367706A5032442574 @default.
• W2000367706 hasAuthorship W2000367706A5052947767 @default.
• W2000367706 hasAuthorship W2000367706A5056111187 @default.
• W2000367706 hasAuthorship W2000367706A5062250759 @default.
• W2000367706 hasAuthorship W2000367706A5064955096 @default.
• W2000367706 hasAuthorship W2000367706A5084771066 @default.
• W2000367706 hasAuthorship W2000367706A5086348397 @default.
• W2000367706 hasConcept C121608353 @default.
• W2000367706 hasConcept C126322002 @default.
• W2000367706 hasConcept C150903083 @default.
• W2000367706 hasConcept C185592680 @default.
• W2000367706 hasConcept C202751555 @default.
• W2000367706 hasConcept C207001950 @default.
• W2000367706 hasConcept C2778250585 @default.
• W2000367706 hasConcept C502942594 @default.
• W2000367706 hasConcept C55493867 @default.
• W2000367706 hasConcept C71924100 @default.
• W2000367706 hasConcept C86803240 @default.
• W2000367706 hasConcept C98274493 @default.
• W2000367706 hasConceptScore W2000367706C121608353 @default.
• W2000367706 hasConceptScore W2000367706C126322002 @default.
• W2000367706 hasConceptScore W2000367706C150903083 @default.
• W2000367706 hasConceptScore W2000367706C185592680 @default.
• W2000367706 hasConceptScore W2000367706C202751555 @default.
• W2000367706 hasConceptScore W2000367706C207001950 @default.
• W2000367706 hasConceptScore W2000367706C2778250585 @default.
• W2000367706 hasConceptScore W2000367706C502942594 @default.
• W2000367706 hasConceptScore W2000367706C55493867 @default.
• W2000367706 hasConceptScore W2000367706C71924100 @default.
• W2000367706 hasConceptScore W2000367706C86803240 @default.
• W2000367706 hasConceptScore W2000367706C98274493 @default.
• W2000367706 hasLocation W20003677061 @default.
• W2000367706 hasOpenAccess W2000367706 @default.
• W2000367706 hasPrimaryLocation W20003677061 @default.
• W2000367706 hasRelatedWork W1997958302 @default.
• W2000367706 hasRelatedWork W2025883670 @default.
• W2000367706 hasRelatedWork W2052327609 @default.
• W2000367706 hasRelatedWork W2057065997 @default.
• W2000367706 hasRelatedWork W2059831422 @default.
• W2000367706 hasRelatedWork W2129633887 @default.
• W2000367706 hasRelatedWork W2134860537 @default.
• W2000367706 hasRelatedWork W2167197150 @default.
• W2000367706 hasRelatedWork W2187456576 @default.
• W2000367706 hasRelatedWork W2198663946 @default.
• W2000367706 hasRelatedWork W2297085063 @default.
• W2000367706 hasRelatedWork W2324086101 @default.
• W2000367706 hasRelatedWork W2351345319 @default.
• W2000367706 hasRelatedWork W2547035050 @default.
• W2000367706 hasRelatedWork W2790292133 @default.
• W2000367706 hasRelatedWork W2891821566 @default.
• W2000367706 hasRelatedWork W2901410810 @default.
• W2000367706 hasRelatedWork W3008079900 @default.
• W2000367706 hasRelatedWork W3086429722 @default.
• W2000367706 hasRelatedWork W3185946424 @default.
• W2000367706 isParatext "false" @default.
• W2000367706 isRetracted "false" @default.
• W2000367706 magId "2000367706" @default.
• W2000367706 workType "article" @default.