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- W2000368527 abstract "Optimal conditions have been determined for the coupling of rat liver phenylalanine hydroxylase (Phe H) to cyanogen bromide-Sepharose 4B. When 8 mg of ligand was reacted with 100 mg of matrix, 20 to 30 percent of the initial enzyme activity was covalently bound along with 90 percent of the protein. The coupled enzyme showed greater thermal stability from 40° to 60°, a broader base of optimal pH activity from 5.8 to 10.0, more resistance to proteolysis and less inhibition by various inhibitors. The uncoupled enzyme exhibited greater storage stability at 25° after 24 hr and at 0° after 18 days. Alteration of the microenvironment by introduction of sulfhydryl groups or of carriers having positive or negative charges had variable effects on the hydroxylase activity." @default.
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- W2000368527 date "1978-07-01" @default.
- W2000368527 modified "2023-09-26" @default.
- W2000368527 title "Covalent coupling of rat liver phenylalanine hydroxylase" @default.
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- W2000368527 doi "https://doi.org/10.1016/0361-9230(78)90107-7" @default.
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