Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000370325> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2000370325 endingPage "11258" @default.
- W2000370325 startingPage "11254" @default.
- W2000370325 abstract "The mycobacterial cell wall contains large amounts of unusual lipids, including mycolic acids that are covalently linked to the underlying arabinogalactan-peptidoglycan complex. Hydrocarbon chains of much of these lipids have been shown to be packed in a direction perpendicular to the plane of the cell surface. In this study, we examined the dynamic properties of the organized lipid domains in the cell wall isolated from Mycobacterium chelonae grown at 30 degrees C. Differential scanning calorimetry showed that much of the lipids underwent major thermal transitions between 30 degree C and 65 degrees C, that is at temperatures above the growth temperature, a result suggesting that a significant portion of the lipids existed in a structure of extremely low fluidity in the growing cells. Spin-labeled fatty acid probes were successfully inserted into the more fluid part of the cell wall. Our model of the cell wall suggests that this domain corresponds to the outermost leaflet, a conclusion reinforced by the observation that labeling of intact cells produced electron spin resonance spectra similar to those of the isolated cell wall. Use of stearate labeled at different positions showed that the fluidity within the outer leaflet increased only slightly as the nitroxide group was placed farther away from the surface. These results are consistent with the model of mycobacterial cell wall containing an asymmetric lipid bilayer, with an internal, less fluid mycolic acid leaflet and an external, more fluid leaflet composed of lipids containing shorter chain fatty acids. The presence of the low-fluidity layer will lower the permeability of the cell wall to lipophilic antibiotics and chemotherapeutic agents and may contribute to the well-known intrinsic resistance of mycobacteria to such compounds." @default.
- W2000370325 created "2016-06-24" @default.
- W2000370325 creator A5019340392 @default.
- W2000370325 creator A5086380170 @default.
- W2000370325 creator A5089381339 @default.
- W2000370325 date "1995-11-21" @default.
- W2000370325 modified "2023-10-14" @default.
- W2000370325 title "Fluidity of the lipid domain of cell wall from Mycobacterium chelonae." @default.
- W2000370325 cites W1635858559 @default.
- W2000370325 cites W1908882478 @default.
- W2000370325 cites W1986073549 @default.
- W2000370325 cites W1997350595 @default.
- W2000370325 cites W2027039061 @default.
- W2000370325 cites W2036317207 @default.
- W2000370325 cites W2050093743 @default.
- W2000370325 cites W2064048353 @default.
- W2000370325 cites W2065934807 @default.
- W2000370325 cites W2088184536 @default.
- W2000370325 cites W2091044472 @default.
- W2000370325 cites W2092898081 @default.
- W2000370325 cites W2100871501 @default.
- W2000370325 cites W2134139057 @default.
- W2000370325 cites W2140981202 @default.
- W2000370325 cites W2142692636 @default.
- W2000370325 cites W2146519942 @default.
- W2000370325 cites W2154690748 @default.
- W2000370325 cites W2163014180 @default.
- W2000370325 cites W2166194168 @default.
- W2000370325 cites W2229737375 @default.
- W2000370325 doi "https://doi.org/10.1073/pnas.92.24.11254" @default.
- W2000370325 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/40610" @default.
- W2000370325 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7479975" @default.
- W2000370325 hasPublicationYear "1995" @default.
- W2000370325 type Work @default.
- W2000370325 sameAs 2000370325 @default.
- W2000370325 citedByCount "133" @default.
- W2000370325 countsByYear W20003703252012 @default.
- W2000370325 countsByYear W20003703252014 @default.
- W2000370325 countsByYear W20003703252016 @default.
- W2000370325 countsByYear W20003703252017 @default.
- W2000370325 countsByYear W20003703252018 @default.
- W2000370325 countsByYear W20003703252019 @default.
- W2000370325 countsByYear W20003703252020 @default.
- W2000370325 countsByYear W20003703252023 @default.
- W2000370325 crossrefType "journal-article" @default.
- W2000370325 hasAuthorship W2000370325A5019340392 @default.
- W2000370325 hasAuthorship W2000370325A5086380170 @default.
- W2000370325 hasAuthorship W2000370325A5089381339 @default.
- W2000370325 hasBestOaLocation W20003703251 @default.
- W2000370325 hasConcept C122398194 @default.
- W2000370325 hasConcept C125235067 @default.
- W2000370325 hasConcept C12554922 @default.
- W2000370325 hasConcept C185592680 @default.
- W2000370325 hasConcept C2776634448 @default.
- W2000370325 hasConcept C2778691952 @default.
- W2000370325 hasConcept C39944091 @default.
- W2000370325 hasConcept C41625074 @default.
- W2000370325 hasConcept C55493867 @default.
- W2000370325 hasConcept C79123411 @default.
- W2000370325 hasConcept C8010536 @default.
- W2000370325 hasConcept C86803240 @default.
- W2000370325 hasConceptScore W2000370325C122398194 @default.
- W2000370325 hasConceptScore W2000370325C125235067 @default.
- W2000370325 hasConceptScore W2000370325C12554922 @default.
- W2000370325 hasConceptScore W2000370325C185592680 @default.
- W2000370325 hasConceptScore W2000370325C2776634448 @default.
- W2000370325 hasConceptScore W2000370325C2778691952 @default.
- W2000370325 hasConceptScore W2000370325C39944091 @default.
- W2000370325 hasConceptScore W2000370325C41625074 @default.
- W2000370325 hasConceptScore W2000370325C55493867 @default.
- W2000370325 hasConceptScore W2000370325C79123411 @default.
- W2000370325 hasConceptScore W2000370325C8010536 @default.
- W2000370325 hasConceptScore W2000370325C86803240 @default.
- W2000370325 hasIssue "24" @default.
- W2000370325 hasLocation W20003703251 @default.
- W2000370325 hasLocation W20003703252 @default.
- W2000370325 hasLocation W20003703253 @default.
- W2000370325 hasLocation W20003703254 @default.
- W2000370325 hasOpenAccess W2000370325 @default.
- W2000370325 hasPrimaryLocation W20003703251 @default.
- W2000370325 hasRelatedWork W1553729 @default.
- W2000370325 hasRelatedWork W1610099743 @default.
- W2000370325 hasRelatedWork W2000370325 @default.
- W2000370325 hasRelatedWork W2033352215 @default.
- W2000370325 hasRelatedWork W2068452135 @default.
- W2000370325 hasRelatedWork W2165404422 @default.
- W2000370325 hasRelatedWork W2171222225 @default.
- W2000370325 hasRelatedWork W2239518055 @default.
- W2000370325 hasRelatedWork W2394562722 @default.
- W2000370325 hasRelatedWork W2464823870 @default.
- W2000370325 hasVolume "92" @default.
- W2000370325 isParatext "false" @default.
- W2000370325 isRetracted "false" @default.
- W2000370325 magId "2000370325" @default.
- W2000370325 workType "article" @default.