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- W2000372103 abstract "By fractional extraction of minced bovine heart muscle with iso-osmotic sucrose and phosphate buffer solutions, it is shown that less than 4% of the total citrate synthase in the tissue is in the cytosol. Using citrate synthase as a marker for broken mitochondria, two methods of fractionation of 750 x g supernatants from homogenates of bovine heart muscle show that 10% of the total fumarase and NADP-linked isocitrate dehydrogenase activities are present in the cytoplasm. Homogenates prepared by sonication and osmotic shock and by sand-grinding gave closely similar results as regards enzyme distributions and extent of mitochondrial breakage. The results are compared with those reported for other tissues." @default.
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- W2000372103 title "Intracellular distribution of NADP-linked isocitrate dehydrogenase, fumarase and citrate synthase in bovine heart muscle" @default.
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- W2000372103 doi "https://doi.org/10.1016/0304-4165(80)90048-3" @default.
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