Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000375983> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2000375983 endingPage "885" @default.
- W2000375983 startingPage "877" @default.
- W2000375983 abstract "The objective of this study is to introduce a new and simple conjugation method of protein with poly(ethylene glycol) (PEG) based on metal coordination. A chelating residue N,N-bis(carboxymethyl)-L-lysine (NTA) was chemically introduced into one terminal of PEG with another terminal of methoxy group (MeO-PEG). Cu2+ or Zn2+ ions were chelated into the NTA residue of MeO-PEG-NTA prepared. Chromatographic experiments revealed that transferrin (Tf) was conjugated to the MeO-PEG-NTA based on Cu2+ ions coordination. The amount of PEG conjugated increased with an increase in that added. Fluorescently labeled Tf was conjugated by the MeO-PEG-NTA with Cu2+ or Zn2+ coordination and injected intravenously into tumor-bearing mice. The Tf conjugated with the MeO-PEG-NTA based on Cu2+ coordination was accumulated in the tumor site to a significantly great extent compared with the free Tf and Tf conjugated with the Zn2+ coordinated MeO-PEG-NTA. A body distribution assay with radiolabeled Tf demonstrated that PEG conjugation with Cu2+ coordination enabled Tf to prolong the blood circulation and enhance the tumor accumulation. When interferon (IFN) was conjugated with the Cu(2+)-coordinated MeO-PEG-NTA and subcutaneously injected into tumor-bearing mice, the in vivo tumor growth was significantly suppressed compared with the injection of free IFN and IFN conjugated by Zn(2+)-coordinated MeO-PEG-NTA. It is concluded that the Cu(2+)-coordinated PEG conjugation can modify the body distribution of protein only by simple mixing in aqueous solution. The PEG conjugation with metal coordination could increase the in vivo half-life period of proteins in the blood, and consequently enhance the accumulation amount at the tumor site." @default.
- W2000375983 created "2016-06-24" @default.
- W2000375983 creator A5055033211 @default.
- W2000375983 creator A5075282917 @default.
- W2000375983 date "2010-02-01" @default.
- W2000375983 modified "2023-10-16" @default.
- W2000375983 title "Tumor Targeting of Protein Through Poly(ethylene glycol) Conjugation with Metal Coordination" @default.
- W2000375983 doi "https://doi.org/10.1166/jnn.2010.1812" @default.
- W2000375983 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20352731" @default.
- W2000375983 hasPublicationYear "2010" @default.
- W2000375983 type Work @default.
- W2000375983 sameAs 2000375983 @default.
- W2000375983 citedByCount "4" @default.
- W2000375983 countsByYear W20003759832015 @default.
- W2000375983 countsByYear W20003759832017 @default.
- W2000375983 countsByYear W20003759832023 @default.
- W2000375983 crossrefType "journal-article" @default.
- W2000375983 hasAuthorship W2000375983A5055033211 @default.
- W2000375983 hasAuthorship W2000375983A5075282917 @default.
- W2000375983 hasConcept C10138342 @default.
- W2000375983 hasConcept C112613896 @default.
- W2000375983 hasConcept C13965031 @default.
- W2000375983 hasConcept C162324750 @default.
- W2000375983 hasConcept C178790620 @default.
- W2000375983 hasConcept C185592680 @default.
- W2000375983 hasConcept C188027245 @default.
- W2000375983 hasConcept C197404232 @default.
- W2000375983 hasConcept C199164860 @default.
- W2000375983 hasConcept C21951064 @default.
- W2000375983 hasConcept C22814914 @default.
- W2000375983 hasConcept C2777516009 @default.
- W2000375983 hasConcept C521977710 @default.
- W2000375983 hasConcept C54400483 @default.
- W2000375983 hasConcept C544153396 @default.
- W2000375983 hasConcept C55493867 @default.
- W2000375983 hasConceptScore W2000375983C10138342 @default.
- W2000375983 hasConceptScore W2000375983C112613896 @default.
- W2000375983 hasConceptScore W2000375983C13965031 @default.
- W2000375983 hasConceptScore W2000375983C162324750 @default.
- W2000375983 hasConceptScore W2000375983C178790620 @default.
- W2000375983 hasConceptScore W2000375983C185592680 @default.
- W2000375983 hasConceptScore W2000375983C188027245 @default.
- W2000375983 hasConceptScore W2000375983C197404232 @default.
- W2000375983 hasConceptScore W2000375983C199164860 @default.
- W2000375983 hasConceptScore W2000375983C21951064 @default.
- W2000375983 hasConceptScore W2000375983C22814914 @default.
- W2000375983 hasConceptScore W2000375983C2777516009 @default.
- W2000375983 hasConceptScore W2000375983C521977710 @default.
- W2000375983 hasConceptScore W2000375983C54400483 @default.
- W2000375983 hasConceptScore W2000375983C544153396 @default.
- W2000375983 hasConceptScore W2000375983C55493867 @default.
- W2000375983 hasIssue "2" @default.
- W2000375983 hasLocation W20003759831 @default.
- W2000375983 hasLocation W20003759832 @default.
- W2000375983 hasOpenAccess W2000375983 @default.
- W2000375983 hasPrimaryLocation W20003759831 @default.
- W2000375983 hasRelatedWork W2000375983 @default.
- W2000375983 hasRelatedWork W2022283669 @default.
- W2000375983 hasRelatedWork W2093329160 @default.
- W2000375983 hasRelatedWork W2147405715 @default.
- W2000375983 hasRelatedWork W2367906838 @default.
- W2000375983 hasRelatedWork W2393146302 @default.
- W2000375983 hasRelatedWork W2412828964 @default.
- W2000375983 hasRelatedWork W4319312373 @default.
- W2000375983 hasRelatedWork W807352614 @default.
- W2000375983 hasRelatedWork W2069367898 @default.
- W2000375983 hasVolume "10" @default.
- W2000375983 isParatext "false" @default.
- W2000375983 isRetracted "false" @default.
- W2000375983 magId "2000375983" @default.
- W2000375983 workType "article" @default.