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- W2000376208 abstract "We have used MLR to assess the cell interactions that enable human PBL stimulated with irradiated, allogeneic PBL to give rise to allospecific, IFN‐γ‐producing CD4 T cells. We were able to generate alloantigen‐specific, IFN‐γ‐producing T cells from peripheral blood. The responder and stimulator cell numbers required for the optimal generation of such cells, enumerated using an enzyme‐linked immunospot assay, were separately determined for various combinations of responders and stimulators. The number of antigen‐specific, IFN‐γ‐producing cells generated per 10 6 responder cells, seeded at the initiation of culture, is critically dependent on the density of the responder cells, with minimal generation of IFN‐γ‐producing T cells at low responder densities. These and further observations with fractionated responding PBL show that CD4 T‐cell/CD4 T‐cell interactions, which are completely independent of CD8 T cells, are necessary for the in vitro generation of alloantigen‐specific, IFN‐γ‐producing CD4 T cells." @default.
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- W2000376208 date "2005-02-28" @default.
- W2000376208 modified "2023-10-15" @default.
- W2000376208 title "Cooperation between CD4 T helper cells is required for the generation of alloantigen‐specific, IFN‐γ‐producing human CD4 T cells" @default.
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- W2000376208 doi "https://doi.org/10.1111/j.1440-1711.2005.01306.x" @default.
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