Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000379733> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W2000379733 endingPage "tw145" @default.
- W2000379733 startingPage "tw145" @default.
- W2000379733 abstract "Tyrosine phosphorylation and activation of phospholipase C-γ (PLC-γ) after stimulation of the T cell antigen receptor (TCR) is dependent on the activities of the tyrosine kinases Lck, ZAP-70 (ζ chain-associated protein kinase of 70 kD), and ITK (IL-2-inducible T cell kinase). Other important components are adaptor molecules, such as SLP-76 (SH2-domain-containing leukocyte protein of 76 kD), which couple Syk family kinases, such as ZAP-70, to PLC-γ activation. Two tyrosine residues important for TCR-mediated phosphorylation and activation of PLC-γ1 are Y783 and Y775. Although previous studies suggest that ZAP-70 and ITK act together to activate PLC-γ1, it is not known which is responsible for phosphorylating the critical tyrosines. Bogin et al. performed studies in a SLP-76-deficient T cell line called J14 (derived from the human CD4+ T cell line, Jurkat) to answer this question. In vitro kinase assays with ITK, but not ZAP-70, immunoprecipitated from TCR-stimulated J14 cells (transfected with a tagged form of SLP-76) resulted in the phosphorylation of both Y783 and Y775 in a recombinant form of PLC-γ1, whereas both kinases could phosphorylate SLP-76. The ability of ITK to phosphorylate PLC-γ1 was dependent on SLP-76. Studies with mutants of SLP-76 found that ITK phosphorylation of recombinant PLC-γ1 was dependent on the presence of tyrosines in the N-terminal region of SLP-76. Coimmunoprecipitation studies revealed that stimulation of the TCR resulted in the association of SLP-76 with ITK in J14 cells. Immunoprecipitated ITK in complex with SLP-76 was catalytically more active than uncomplexed ITK. These data suggest that SLP-76 is necessary to initiate and maintain the activity of ITK." @default.
- W2000379733 created "2016-06-24" @default.
- W2000379733 creator A5040603302 @default.
- W2000379733 date "2007-04-17" @default.
- W2000379733 modified "2023-09-25" @default.
- W2000379733 title "Giving ITK the SLP" @default.
- W2000379733 doi "https://doi.org/10.1126/stke.3832007tw145" @default.
- W2000379733 hasPublicationYear "2007" @default.
- W2000379733 type Work @default.
- W2000379733 sameAs 2000379733 @default.
- W2000379733 citedByCount "0" @default.
- W2000379733 crossrefType "journal-article" @default.
- W2000379733 hasAuthorship W2000379733A5040603302 @default.
- W2000379733 hasConcept C104317684 @default.
- W2000379733 hasConcept C11960822 @default.
- W2000379733 hasConcept C153911025 @default.
- W2000379733 hasConcept C179464577 @default.
- W2000379733 hasConcept C183786373 @default.
- W2000379733 hasConcept C184235292 @default.
- W2000379733 hasConcept C19317047 @default.
- W2000379733 hasConcept C197153747 @default.
- W2000379733 hasConcept C203014093 @default.
- W2000379733 hasConcept C2776090121 @default.
- W2000379733 hasConcept C2777553839 @default.
- W2000379733 hasConcept C35174551 @default.
- W2000379733 hasConcept C42362537 @default.
- W2000379733 hasConcept C55493867 @default.
- W2000379733 hasConcept C62478195 @default.
- W2000379733 hasConcept C71829478 @default.
- W2000379733 hasConcept C86803240 @default.
- W2000379733 hasConcept C8891405 @default.
- W2000379733 hasConcept C95444343 @default.
- W2000379733 hasConceptScore W2000379733C104317684 @default.
- W2000379733 hasConceptScore W2000379733C11960822 @default.
- W2000379733 hasConceptScore W2000379733C153911025 @default.
- W2000379733 hasConceptScore W2000379733C179464577 @default.
- W2000379733 hasConceptScore W2000379733C183786373 @default.
- W2000379733 hasConceptScore W2000379733C184235292 @default.
- W2000379733 hasConceptScore W2000379733C19317047 @default.
- W2000379733 hasConceptScore W2000379733C197153747 @default.
- W2000379733 hasConceptScore W2000379733C203014093 @default.
- W2000379733 hasConceptScore W2000379733C2776090121 @default.
- W2000379733 hasConceptScore W2000379733C2777553839 @default.
- W2000379733 hasConceptScore W2000379733C35174551 @default.
- W2000379733 hasConceptScore W2000379733C42362537 @default.
- W2000379733 hasConceptScore W2000379733C55493867 @default.
- W2000379733 hasConceptScore W2000379733C62478195 @default.
- W2000379733 hasConceptScore W2000379733C71829478 @default.
- W2000379733 hasConceptScore W2000379733C86803240 @default.
- W2000379733 hasConceptScore W2000379733C8891405 @default.
- W2000379733 hasConceptScore W2000379733C95444343 @default.
- W2000379733 hasIssue "383" @default.
- W2000379733 hasLocation W20003797331 @default.
- W2000379733 hasOpenAccess W2000379733 @default.
- W2000379733 hasPrimaryLocation W20003797331 @default.
- W2000379733 hasRelatedWork W1996331566 @default.
- W2000379733 hasRelatedWork W2074885149 @default.
- W2000379733 hasRelatedWork W2077648546 @default.
- W2000379733 hasRelatedWork W2104636614 @default.
- W2000379733 hasRelatedWork W2120071583 @default.
- W2000379733 hasRelatedWork W2127080319 @default.
- W2000379733 hasRelatedWork W2980054351 @default.
- W2000379733 hasRelatedWork W3107681436 @default.
- W2000379733 hasRelatedWork W3172183594 @default.
- W2000379733 hasRelatedWork W4313383272 @default.
- W2000379733 hasVolume "2007" @default.
- W2000379733 isParatext "false" @default.
- W2000379733 isRetracted "false" @default.
- W2000379733 magId "2000379733" @default.
- W2000379733 workType "article" @default.