Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000380030> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2000380030 endingPage "35" @default.
- W2000380030 startingPage "32" @default.
- W2000380030 abstract "A previous study reported the cloning of a putative Prevotella nigrescens-specific DNA probe, Pn23, using random shotgun method. The present study evaluated the species-specificity of Pn23 for P. nigrescens using the clinical strains of Prevotella intermedia and P. nigrescens to develop P. nigrescens-specific polymerase chain reaction (PCR) primers. Southern blot analysis showed that the DNA probe, Pn23, detected only the genomic DNA of P. nigrescens strains. PCR showed that the two sets of PCR primers, Pn23-F1/Pn23-R1 and Pn23-F2/Pn23-R2, had species-specificity for P. nigrescens. Interestingly, the two sets of PCR primers, Pn23-F6/Pn23-R6 and Pn23-F7/Pn23-R7, had strain-specificity for P. nigrescens ATCC 33563. The detection limits of the four primer sets were 40 or 4 pg of the purified genomic DNA of P. nigrescens ATCC 33563. These results suggest that the DNA probe, Pn23, and the two sets of PCR primers, Pn23-F1/Pn23-R1 and Pn23-F2/Pn23-R2, can be useful for the detection of P. nigrescens in the molecular epidemiological studies of oral infectious diseases." @default.
- W2000380030 created "2016-06-24" @default.
- W2000380030 creator A5027381296 @default.
- W2000380030 creator A5049799625 @default.
- W2000380030 creator A5069041659 @default.
- W2000380030 creator A5071498839 @default.
- W2000380030 date "2011-02-01" @default.
- W2000380030 modified "2023-09-25" @default.
- W2000380030 title "Development of Prevotella nigrescens-specific PCR primers based on the nucleotide sequence of a Pn23 DNA probe" @default.
- W2000380030 cites W1571487122 @default.
- W2000380030 cites W1916913548 @default.
- W2000380030 cites W1964957391 @default.
- W2000380030 cites W1968629917 @default.
- W2000380030 cites W1970907020 @default.
- W2000380030 cites W1973130896 @default.
- W2000380030 cites W1973536681 @default.
- W2000380030 cites W1980323457 @default.
- W2000380030 cites W2012465358 @default.
- W2000380030 cites W2047925303 @default.
- W2000380030 cites W2059050422 @default.
- W2000380030 cites W2107597310 @default.
- W2000380030 cites W2119764574 @default.
- W2000380030 cites W2146192212 @default.
- W2000380030 cites W2147994976 @default.
- W2000380030 cites W2158877985 @default.
- W2000380030 doi "https://doi.org/10.1016/j.anaerobe.2010.12.005" @default.
- W2000380030 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21184839" @default.
- W2000380030 hasPublicationYear "2011" @default.
- W2000380030 type Work @default.
- W2000380030 sameAs 2000380030 @default.
- W2000380030 citedByCount "1" @default.
- W2000380030 countsByYear W20003800302014 @default.
- W2000380030 crossrefType "journal-article" @default.
- W2000380030 hasAuthorship W2000380030A5027381296 @default.
- W2000380030 hasAuthorship W2000380030A5049799625 @default.
- W2000380030 hasAuthorship W2000380030A5069041659 @default.
- W2000380030 hasAuthorship W2000380030A5071498839 @default.
- W2000380030 hasConcept C104317684 @default.
- W2000380030 hasConcept C133307689 @default.
- W2000380030 hasConcept C153911025 @default.
- W2000380030 hasConcept C17757408 @default.
- W2000380030 hasConcept C178790620 @default.
- W2000380030 hasConcept C185592680 @default.
- W2000380030 hasConcept C200658500 @default.
- W2000380030 hasConcept C2777563447 @default.
- W2000380030 hasConcept C2780423288 @default.
- W2000380030 hasConcept C49105822 @default.
- W2000380030 hasConcept C51679486 @default.
- W2000380030 hasConcept C523546767 @default.
- W2000380030 hasConcept C54355233 @default.
- W2000380030 hasConcept C552990157 @default.
- W2000380030 hasConcept C86803240 @default.
- W2000380030 hasConcept C89423630 @default.
- W2000380030 hasConcept C90583042 @default.
- W2000380030 hasConceptScore W2000380030C104317684 @default.
- W2000380030 hasConceptScore W2000380030C133307689 @default.
- W2000380030 hasConceptScore W2000380030C153911025 @default.
- W2000380030 hasConceptScore W2000380030C17757408 @default.
- W2000380030 hasConceptScore W2000380030C178790620 @default.
- W2000380030 hasConceptScore W2000380030C185592680 @default.
- W2000380030 hasConceptScore W2000380030C200658500 @default.
- W2000380030 hasConceptScore W2000380030C2777563447 @default.
- W2000380030 hasConceptScore W2000380030C2780423288 @default.
- W2000380030 hasConceptScore W2000380030C49105822 @default.
- W2000380030 hasConceptScore W2000380030C51679486 @default.
- W2000380030 hasConceptScore W2000380030C523546767 @default.
- W2000380030 hasConceptScore W2000380030C54355233 @default.
- W2000380030 hasConceptScore W2000380030C552990157 @default.
- W2000380030 hasConceptScore W2000380030C86803240 @default.
- W2000380030 hasConceptScore W2000380030C89423630 @default.
- W2000380030 hasConceptScore W2000380030C90583042 @default.
- W2000380030 hasIssue "1" @default.
- W2000380030 hasLocation W20003800301 @default.
- W2000380030 hasLocation W20003800302 @default.
- W2000380030 hasOpenAccess W2000380030 @default.
- W2000380030 hasPrimaryLocation W20003800301 @default.
- W2000380030 hasRelatedWork W1490135968 @default.
- W2000380030 hasRelatedWork W169075148 @default.
- W2000380030 hasRelatedWork W1961077450 @default.
- W2000380030 hasRelatedWork W2004027931 @default.
- W2000380030 hasRelatedWork W2021614264 @default.
- W2000380030 hasRelatedWork W2051609464 @default.
- W2000380030 hasRelatedWork W2080534576 @default.
- W2000380030 hasRelatedWork W2101116249 @default.
- W2000380030 hasRelatedWork W2403090579 @default.
- W2000380030 hasRelatedWork W2064206660 @default.
- W2000380030 hasVolume "17" @default.
- W2000380030 isParatext "false" @default.
- W2000380030 isRetracted "false" @default.
- W2000380030 magId "2000380030" @default.
- W2000380030 workType "article" @default.